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Biodistribution of p-iodobenzoyl (PIP) labeled antibodies in a murine lymphoma model

Authors :
Irwin D. Bernstein
Christopher C. Badger
Stephen W. Hadley
Alan R. Fritzberg
D. Scott Wilbur
Source :
International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology. 17(4)
Publication Year :
1990

Abstract

A monoclonal antibody against the murine T-cell antigen Thy 1.1 was radioiodinated using N -succin-imidyl p -iodobenzoate (PIP) in an attempt to decrease deiodination of the labeled antibody. The biodistribution of the PIP labeled antibody was compared to Iodogen labeled antibody in Thy 1.1 + lymphoma bearing AKR/Cum mice, where the antibody was tumor specific, and AKR/J mice where the antibody reacted with both tumor and normal T-cells. PIP labeling resulted in decreased iodine concentrations in stomach and salivary gland as compared to Iodogen labeling. There was little difference in radioiodine concentrations between the two preparations in tumor, lymphoid tissues or other organs. These results suggest deiodination of intact antibody plays little role in the clearance of radioiodinated anti-Thy 1.1 antibody from tissues.

Details

ISSN :
08832897
Volume :
17
Issue :
4
Database :
OpenAIRE
Journal :
International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology
Accession number :
edsair.doi.dedup.....8946492db647ebd524183275fb08980b