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Protein phosphatase 6 is a key factor regulating spermatogenesis
- Source :
- Cell Death Differ, Cell death and differentiation, vol 27, iss 6
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Protein phosphatase 6 (PP6) is a member of the PP2A-like subfamily, which plays a critical role in many fundamental cellular processes. We recently reported that PP6 is essential for female fertility. Here, we report that PP6 is involved in meiotic recombination and that germ cell-specific deletion of PP6 by Stra8-Cre causes defective spermatogenesis. The PP6-deficient spermatocytes were arrested at the pachytene stage and defects in DSB repair and crossover formation were observed, indicating that PP6 facilitated meiotic double-stranded breaks (DSB) repair. Further investigations revealed that depletion of PP6 in the germ cells affected chromatin relaxation, which was dependent on MAPK pathway activity, consequently preventing programmed DSB repair factors from being recruited to proper positions on the chromatin. Taken together, our results demonstrate that PP6 has an important role in meiotic recombination and male fertility.
- Subjects :
- Male
0301 basic medicine
MAPK/ERK pathway
Biochemistry & Molecular Biology
Subfamily
DNA Repair
1.1 Normal biological development and functioning
Phosphatase
Biology
Inbred C57BL
Medical and Health Sciences
Article
Double-Stranded
Mice
03 medical and health sciences
0302 clinical medicine
Meiosis
Underpinning research
Spermatocytes
Genetics
Phosphoprotein Phosphatases
Animals
DNA Breaks, Double-Stranded
Spermatogenesis
Molecular Biology
Contraception/Reproduction
DNA Breaks
Correction
Cell Biology
Biological Sciences
Chromatin
Cell biology
Mice, Inbred C57BL
030104 developmental biology
Male fertility
030220 oncology & carcinogenesis
Generic health relevance
Pachytene Stage
Homologous recombination
Subjects
Details
- ISSN :
- 14765403 and 13509047
- Volume :
- 27
- Database :
- OpenAIRE
- Journal :
- Cell Death & Differentiation
- Accession number :
- edsair.doi.dedup.....8915ea551b24d223341e85e5d95c1a06