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MAPK-mediated auxin signal transduction pathways regulate the malic acid secretion under aluminum stress in wheat (Triticum aestivum L.)
- Source :
- Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017), Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Portfolio, 2017.
-
Abstract
- An isobaric tags for relative and absolute quantitative (iTRAQ)-based quantitative proteomic approach was used to screen the differentially expressed proteins during control treatment (CK), aluminum (Al) and Al+ indole-3-acetic acid (IAA) treatment of wheat lines ET8 (Al-tolerant). Further, the the expression levels of auxin response factor (ARF), Aux/IAA, Mitogen activated protein kinase (MAPK) 2c, and MAPK1a were analyzed. Results showed that 16 proteins were determined to be differentially expressed in response to Al and IAA co-treatment compared with Al alone. Among them, MAPK2c and MAPK1a proteins displayed markedly differential expression during the processes. The expression of ARF2 was upregulated and Aux/IAA was downregulated by Al, while both in concentration- and time-dependent manners. Western-blot detection of MAPK2c and MAPK1a indicated that Al upregulated MAPK2c and downregulated MAPK1a in both concentration- and time-dependent manners. Exogenous IAA could promote the expression of MAPK2c, but inhibit the expression of MAPK1a in the presence/absence of Al. These findings indicated that IAA acted as one of the key signaling molecule controls the response mechanism of wheat malic acid efflux to Al stress through the suppression/activation of Aux/IAA and ARFs, and the activity of MAPK2c and MAPK1a were positively or negatively regulated.
- Subjects :
- 0106 biological sciences
0301 basic medicine
MAPK/ERK pathway
Science
Malates
Plant Roots
01 natural sciences
Arsenicals
Article
03 medical and health sciences
chemistry.chemical_compound
Downregulation and upregulation
Gene Expression Regulation, Plant
Stress, Physiological
heterocyclic compounds
Protein Kinase Inhibitors
Triticum
Plant Proteins
Malic acid secretion
Regulation of gene expression
Multidisciplinary
Indoleacetic Acids
biology
food and beverages
Cell biology
030104 developmental biology
chemistry
Biochemistry
RNA, Plant
Isotope Labeling
Mitogen-activated protein kinase
biology.protein
Medicine
Malic acid
Efflux
Mitogen-Activated Protein Kinases
Signal transduction
Aluminum
Signal Transduction
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....891180fc3295036e5b51e516bdd21db6