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The cell-permeable Aβ1-6A2VTAT(D) peptide reverts synaptopathy induced by Aβ1-42wt
- Source :
- Neurobiology of Disease, Vol 89, Iss, Pp 101-111 (2016)
- Publication Year :
- 2015
-
Abstract
- Alzheimer disease (AD) is the most prevalent form of dementia. Loss of hippocampal synapses is the first neurodegenerative event in AD. Synaptic loss has been associated with the accumulation in the brain parenchyma of soluble oligomeric forms of amyloid β peptide (Aβ1-42wt). Clinical observations have shown that a mutation in the APP protein (A673V) causes an early onset AD-type dementia in homozygous carriers while heterozygous carriers are unaffected. This mutation leads to the formation of mutated Aβ peptides (Aβ1-42A2V) in homozygous patients, while in heterozygous subjects both Aβ1-42wt and Aβ1-42A2V are present. To better understand the impact of the A673V mutation in AD, we analyzed the synaptotoxic effect of oligomers formed by aggregation of different Aβ peptides (Aβ1-42wt or Aβ1-42A2V) and the combination of the two Aβ1-42MIX (Aβ1-42wt and Aβ1-42A2V) in an in vitro model of synaptic injury. We showed that Aβ1-42A2V oligomers are more toxic than Aβ1-42wt oligomers in hippocampal neurons, confirming the results previously obtained in cell lines. Furthermore, we reported that oligomers obtained by the combination of both wild type and mutated peptides (Aβ1-42MIX) did not exert synaptic toxicity. We concluded that the combination of Aβ1-42wt and Aβ1-42A2V peptides hinders the toxicity of Aβ1-42A2V and counteracts the manifestation of synaptopathy in vitro. Finally we took advantage of this finding to generate a cell-permeable peptide for clinical application, by fusing the first six residues of the Aβ1-42A2V to the TAT cargo sequence (Aβ1-6A2VTAT(D)). Noteworthy, the treatment with Aβ1-6A2VTAT(D) confers neuroprotection against both in vitro and in vivo synaptopathy models. Therefore Aβ1-6A2VTAT(D) may represent an innovative therapeutic tool to prevent synaptic degeneration in AD.
- Subjects :
- 0301 basic medicine
Cell Membrane Permeability
Aβ oligomers
Synaptic injury
Aβ oligomer
Dendritic Spines
Peptide
Mice, Transgenic
Biology
medicine.disease_cause
Neuroprotection
Hippocampus
lcsh:RC321-571
03 medical and health sciences
Mice
In vivo
Alzheimer Disease
Brainbow hippocampal neurons
medicine
Animals
lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry
chemistry.chemical_classification
Neurons
Mutation
Cell-permeable peptide
Amyloid beta-Peptides
Wild type
Long-term potentiation
Alzheimer's disease
medicine.disease
Peptide Fragments
Cell biology
Disease Models, Animal
030104 developmental biology
Biochemistry
chemistry
Brainbow hippocampal neuron
Neurology
Synapses
Synaptopathy
A673V mutation
Subjects
Details
- ISSN :
- 1095953X
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Neurobiology of disease
- Accession number :
- edsair.doi.dedup.....88f653e4cae9c92a265f184a4994a082