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Neoplastic transformation of rat thyroid cells requires the junB and fra-1 gene induction which is dependent on the HMGI-C gene product
- Source :
- EMBO journal, (1997)., info:cnr-pdr/source/autori:Vallone D, Battista S, Pierantoni GM, Fedele M, Casalino L, Santoro M, Viglietto G, Fusco A, Verde P./titolo:Neoplastic transformation of rat thyroid cells requires the junB and fra-1 gene induction which is dependent on the HMGI-C gene product./doi:/rivista:EMBO journal (Print)/anno:1997/pagina_da:/pagina_a:/intervallo_pagine:/volume, 16 (1997): 5310–5321. doi:10.1093/emboj/16.17.5310, info:cnr-pdr/source/autori:Vallone, Daniela R.; Battista, Sabrina; Pierantoni, Giovanna Maria; Fedele, Monica; Casalino, Laura; Santoro, Massimo Mattia; Viglietto, Giuseppe; Fusco, Alfredo; Verde, Pasquale/titolo:Neoplastic transformation of rat thyroid cells requires the junB and fra-1 gene induction which is dependent on the HMGI-C gene product/doi:10.1093%2Femboj%2F16.17.5310/rivista:EMBO journal (Print)/anno:1997/pagina_da:5310/pagina_a:5321/intervallo_pagine:5310–5321/volume:16
- Publication Year :
- 1997
- Publisher :
- Wiley, 1997.
-
Abstract
- The expression of the high mobility group I (HMGI)-C chromatin component was shown previously to be essential for the establishment of the neoplastic phenotype in retrovirally transformed thyroid cell lines. To identify possible targets of the HMGI-C gene product, we have analyzed the AP-1 complex in normal, fully transformed and antisense HMGI-C-expressing rat thyroid cells. We show that neoplastic transformation is associated with a drastic increase in AP-1 activity, which reflects multiple compositional changes. The strongest effect is represented by the dramatic junB and fra-1 gene induction, which is prevented in cell lines expressing the antisense HMGI-C. These results indicate that the HMGI-C gene product is essential for the junB and fra-1 transcriptional induction associated with neoplastic transformation. The inhibition of Fra-1 protein synthesis by stable transfection with a fra-1 antisense RNA vector significantly reduces the malignant phenotype of the transformed thyroid cells, indicating a pivotal role for the fra-1 gene product in the process of cellular transformation.
- Subjects :
- Transcriptional Activation
HMGI
Proto-Oncogene Proteins c-jun
JUNB
Thyroid Gland
Biology
General Biochemistry, Genetics and Molecular Biology
thyroid
fra-1
Gene product
medicine
Protein biosynthesis
Animals
RNA, Antisense
Neoplastic transformation
RNA, Messenger
RNA, Neoplasm
Thyroid Neoplasms
AP1
Molecular Biology
General Immunology and Microbiology
General Neuroscience
HMGA2 Protein
Thyroid
High Mobility Group Proteins
AP-1
Phenotype
Molecular biology
Rats
Chromatin
Antisense RNA
Cell biology
Gene Expression Regulation, Neoplastic
Transcription Factor AP-1
Cell Transformation, Neoplastic
medicine.anatomical_structure
Proto-Oncogene Proteins c-fos
Research Article
Protein Binding
Subjects
Details
- ISSN :
- 14602075
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- The EMBO Journal
- Accession number :
- edsair.doi.dedup.....88f61cba24b1941e06566336477d3522