Additional file 2: of Differential effects of the Akt inhibitor MK-2206 on migration and radiation sensitivity of glioblastoma cells

Table S1. Cell cycle-phase distribution in control and irradiated (2 or 8 Gy) SNB19 tumor cells. The cells were fixed either 30 min or 24 h after IR, permeabilized, stained with propidium iodide, and analyzed for their DNA content by flow cytometry. Data are presented as means (± SD) from at least three independent experiments. For detailed description, see legend to Fig. 3. Table S2. Detection of γH2AX as a measure of DNA damage in SNB19 cells by flow cytometry. Mean γH2AX values are normalized to the non-irradiated control (30 min post-irradiation). The data are means ±SE from at least three independent experiments. Table S3. Cloning efficiencies and radiosensitivity parametersa of in vitro irradiated tumor cell lines untreated and pretreated with the MK-2206 and PI-103 either alone or in combination. aMean (± SE) from at least three independent experiments; bCF2 is the colony-forming ability at 2 Gy; cD10 is the radiation dose required to reduce colony-forming ability by 10%; dThe growth inhibition factor IF10 was calculated as (D10 control)/(D10 + inh.). Table S4. Impact of MK-2206, PI-103 either alone or in combination on the area-specific plasma membrane capacitance Cm, the whole-cell capacitance CC* and cell radius. *The data derived from the ROT experiments shown in the Additional file 4: Figure S10 represent the means ± SE of at least 60 cells. (DOCX 63 kb)