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Chlorophyll Oxidative Metabolism During the Phototrophic and Heterotrophic Growth of Scenedesmus obliquus
- Source :
- Antioxidants, Volume 8, Issue 12, Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- 15 Páginas.-- 5 Tablas.-- 4 Figuras<br />Different cultivation strategies have been developed with the aim of increasing the production rate of microalgal pigments. Specifically, biotechnological approaches are designed to increase antioxidant metabolites as chlorophyll and carotenoids. However, although significant advances have been built up, available information regarding both the chlorophyll metabolism and their oxidative reactions in photobioreactors is scarce. To unravel such processes, the detailed chlorophyll and carotenoid fraction of Scenedesmus obliquus has been studied by HPLC-ESI/APCI-hrTOF-MS from phototrophic and heterotrophic cultures. Scenedesmus is provided with a controlled strategy of interconversion between chlorophyll a and b to avoid the formation of reactive oxygen species (ROS) at high irradiances in addition to the photoacclimation of carotenoids. Indeed, precise kinetics of 132-hydroxy- and 151-hydroxy-lactone chlorophyll metabolites shows the existence of a chlorophyll oxidative metabolism as a tool to manage the excess of energy at high light conditions. Unexpectedly, the oxidation under phototrophy favored chlorophyll b metabolites over the chlorophyll a series, while the heterotrophic conditions exclusively induced the formation of 132-hydroxy-chlorophyll a. In parallel, during the first 48 h of growth in the dark, the chlorophyll fraction maintained a promising steady state. Although future studies are required to resolve the biochemical reactions implied in the chlorophyll oxidative metabolism, the present results agree with phytoplankton metabolism.<br />This work was supported by the Ministerio de Ciencia, Investigación y Universidades, Agencia Estatal de Investigación y Fondo Europeo de Desarrollo Regional (FEDER), grant number RTI2018-095415-B-I00. MM was supported with a fellowship from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 and the Brazilian Funding Agency FAPERGS (Fundação de Amparo a pesquisa do estado do Rio Grande do Sul). The APC was partially funded by CSIC.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Chlorophyll b
chlorophylls
Chlorophyll a
Photoacclimation
Physiology
Clinical Biochemistry
phototrophic
lactone-chlorophyll
hydroxy-chlorophyll
Photobioreactor
01 natural sciences
Biochemistry
Article
Scenedesmus
03 medical and health sciences
chemistry.chemical_compound
Lactone-chlorophyll
oxidative metabolism
Phytoplankton
Molecular Biology
Carotenoid
Phototrophic
chemistry.chemical_classification
biology
Phototroph
carotenoids
photoacclimation
ROS
Cell Biology
Chlorophylls
biology.organism_classification
Carotenoids
030104 developmental biology
chemistry
Hydroxy-chlorophyll
Chlorophyll
Oxidative metabolism
Heterotrophic
heterotrophic
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 20763921
- Database :
- OpenAIRE
- Journal :
- Antioxidants
- Accession number :
- edsair.doi.dedup.....88c82b7d753bddc56be9d0cb33d124d2
- Full Text :
- https://doi.org/10.3390/antiox8120600