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During Adipocyte Remodeling, Lipid Droplet Configurations Regulate Insulin Sensitivity through F-Actin and G-Actin Reorganization
- Source :
- Molecular and cellular biology. 39(20)
- Publication Year :
- 2019
-
Abstract
- Adipocytes have unique morphological traits in insulin sensitivity control. However, how the appearance of adipocytes can determine insulin sensitivity has not been understood. Here, we demonstrate that actin cytoskeleton reorganization upon lipid droplet (LD) configurations in adipocytes plays important roles in insulin-dependent glucose uptake by regulating GLUT4 trafficking. Compared to white adipocytes, brown/beige adipocytes with multilocular LDs exhibited well-developed filamentous actin (F-actin) structure and potentiated GLUT4 translocation to the plasma membrane in the presence of insulin. In contrast, LD enlargement and unilocularization in adipocytes downregulated cortical F-actin formation, eventually leading to decreased F-actin-to-globular actin (G-actin) ratio and suppression of insulin-dependent GLUT4 trafficking. Pharmacological inhibition of actin polymerization accompanied with impaired F/G-actin dynamics reduced glucose uptake in adipose tissue and conferred systemic insulin resistance in mice. Thus, our study reveals that adipocyte remodeling with different LD configurations could be an important factor to determine insulin sensitivity by modulating F/G-actin dynamics.
- Subjects :
- Male
medicine.medical_treatment
Adipocytes, White
030209 endocrinology & metabolism
macromolecular substances
Filamentous actin
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Insulin resistance
Lipid droplet
Adipocyte
medicine
Adipocytes
Animals
Obesity
Cytoskeleton
Molecular Biology
030304 developmental biology
0303 health sciences
Glucose Transporter Type 4
biology
Insulin
Cold-Shock Response
Gene Expression Profiling
Actin cytoskeleton reorganization
Cell Biology
Lipid Droplets
medicine.disease
Actins
Cell biology
Mice, Inbred C57BL
Actin Cytoskeleton
Protein Transport
Glucose
chemistry
Adipose Tissue
Gene Expression Regulation
biology.protein
Insulin Resistance
GLUT4
Research Article
Subjects
Details
- ISSN :
- 10985549
- Volume :
- 39
- Issue :
- 20
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular biology
- Accession number :
- edsair.doi.dedup.....88b9f58457fe8884a3afc57096fb0f77