Additional file 4: of Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential

Figure S2. (A) Representative flow cytometry graphs of the negative controls, isotype controls, and ABCG2-stained hPSC-LSC samples in different time points. (B) Morphology and ABCG2/p63α expression of day 11 sorted ABCG2-positive hPSC-LSCs after continued culture (17 days) in CnT-30 medium and on LN-521/Col IV (B). Scale bar, 100 μm. Cell nuclei counterstained with DAPI (blue). BF: brightfield, FACS: fluorescence-activated cell sorting. Data are presented with the representative hESC line Regea08/017. (DOCX 1668 kb)