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HIV-1 enhancing effect of prostatic acid phosphatase peptides is reduced in human seminal plasma
- Source :
- PLoS ONE, Vol 6, Iss 1, p e16285 (2011), PLoS ONE, PLoS ONE; 6(1) (2011)
- Publication Year :
- 2011
- Publisher :
- Public Library of Science (PLoS), 2011.
-
Abstract
- We recently reported that HIV-1 infection can be inhibited by innate antimicrobial components of human seminal plasma (SP). Conversely, naturally occurring peptidic fragments from the SP-derived prostatic acid phosphatase ( PAP) have been reported to form amyloid fibrils called "SEVI'' and enhance HIV-1 infection in vitro. In order to understand the biological consequence of this proviral effect, we extended these studies in the presence of human SP. PAP-derived peptides were agitated to form SEVI and incubated in the presence or absence of SP. While PAP-derived peptides and SEVI alone were proviral, the presence of 1% SP ablated their proviral activity in several different anti-HIV-1 assays. The anti-HIV-1 activity of SP was concentration dependent and was reduced following filtration. Supraphysiological concentrations of PAP peptides and SEVI incubated with diluted SP were degraded within hours, with SP exhibiting proteolytic activity at dilutions as high as 1:200. Sub-physiological concentrations of two prominent proteases of SP, prostate-specific antigen (PSA) and matriptase, could degrade physiological and supraphysiological concentrations of PAP peptides and SEVI. While human SP is a complex biological fluid, containing both antiviral and proviral factors, our results suggest that PAP peptides and SEVI may be subject to naturally occurring proteolytic components capable of reducing their proviral activity.
- Subjects :
- Proteases
Infectious Medicine
Amyloid
Anatomy and Physiology
Acid Phosphatase
Immunology
lcsh:Medicine
Protein tyrosine phosphatase
Microbiology
03 medical and health sciences
Antigen
Semen
Virology
Humans
Matriptase
MTT assay
lcsh:Science
Biology
030304 developmental biology
0303 health sciences
Multidisciplinary
HIV Enhancer
biology
Dose-Response Relationship, Drug
030302 biochemistry & molecular biology
Serine Endopeptidases
lcsh:R
Acid phosphatase
Immunity
Prostate-Specific Antigen
Antivirals
Molecular biology
In vitro
Peptide Fragments
Innate Immunity
3. Good health
Host-Pathogen Interaction
Biochemistry
Prostatic acid phosphatase
biology.protein
HIV-1
Medicine
Clinical Immunology
lcsh:Q
Fluid Physiology
Protein Tyrosine Phosphatases
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 6
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....8896dbbc7f0e32731524691df82699ca