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Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea
- Source :
- The Korean journal of parasitology. 41(3)
- Publication Year :
- 2003
-
Abstract
- Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a well-known virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T. gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T. gondii isolate is reported for the first time in the Republic of Korea.
- Subjects :
- Virulence
Antigens, Protozoan
Parasitemia
Mice
Antigen
Serial passage
parasitic diseases
medicine
Tumor Cells, Cultured
Parasite hosting
Animals
Humans
Serial Passage
Sarcoma 180
Toxoplasmosis, Ocular
Infectivity
Mice, Inbred BALB C
Korea
biology
Toxoplasma gondii
Middle Aged
medicine.disease
biology.organism_classification
Virology
Toxoplasmosis
Specific Pathogen-Free Organisms
Microscopy, Electron
Infectious Diseases
Parasitology
Female
Original Article
Toxoplasma
Encephalitis
Subjects
Details
- ISSN :
- 00234001
- Volume :
- 41
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- The Korean journal of parasitology
- Accession number :
- edsair.doi.dedup.....888857f4236e224fa8d5d8becde1c81b