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miR-539 acts as a tumor suppressor by targeting epidermal growth factor receptor in breast cancer
- Source :
- Scientific Reports, Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Breast cancer is the most frequently diagnosed malignancy and the leading cause of cancer-associated death in women worldwide. microRNAs (miRNAs) play critical roles in the cellular processes of breast cancer. However, the crucial roles and underlying mechanisms of miR-539 in breast cancer remain unclear. By RT-qPCR, we found that expression of miR-539 was markedly down-regulated in breast cancer tissues and cell lines compared with that in paired adjacent normal tissues and normal cell lines. The low level of miR-539 expression was positively associated with lymph node metastasis. Furthermore, forced expression of miR-539 inhibited proliferation and migration of breast cancer MDA-MB-231 and MCF7 cells in vitro and suppressed tumor growth in vivo. Moreover, bioinformatics analysis and luciferase reporter assays indicated that epidermal growth factor receptor (EGFR) was a direct target of miR-539. Over-expression of miR-539 decreased the EGFR mRNA and protein levels in MDA-MB-231 and MCF7 cells. In addition, ectopic over-expression of EGFR partly reversed miR-539-inhibited proliferation as well as migration of MDA-MB-231 and MCF7 cells. Taken together, our results demonstrate that miR-539 functions as a tumor suppressor in breast cancer by downregulating EGFR, supporting the targeting of the novel miR-539/EGFR axis as a potentially effective therapeutic approach for breast cancer.
- Subjects :
- Adult
Male
0301 basic medicine
lcsh:Medicine
Down-Regulation
Mice, Nude
Breast Neoplasms
Malignancy
Article
law.invention
03 medical and health sciences
Breast cancer
Downregulation and upregulation
Cell Movement
law
microRNA
medicine
Animals
Humans
Epidermal growth factor receptor
lcsh:Science
skin and connective tissue diseases
Cell Proliferation
Multidisciplinary
biology
Cell growth
lcsh:R
Middle Aged
medicine.disease
ErbB Receptors
MicroRNAs
030104 developmental biology
Cell culture
MCF-7 Cells
Cancer research
biology.protein
Suppressor
lcsh:Q
Female
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....8864a72f9f569a74f25ef8961c55581f