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Construction and characterization of aPorphyromonas gingivalis htpGdisruption mutant
- Source :
- FEMS Microbiology Letters. 225:101-106
- Publication Year :
- 2003
- Publisher :
- Oxford University Press (OUP), 2003.
-
Abstract
- Our previous reports implicated the Hsp90 homologue (HtpG) of Porphyromonas gingivalis (Pg) in its virulence in periodontal disease. We investigated the role of the HtpG stress protein in the virulence of Pg. This report describes the (i) expression of a recombinant Pg HtpG (rHtpG), (ii) generation and characterization of a polyclonal rabbit anti-Pg rHtpG antiserum, and (iii) construction of a Pg htpG isogenic mutant and evaluation of the growth, adherence and invasion properties compared to the wild-type parental strain. The disruption of the htpG gene did not significantly affect growth, and had no effect on Pg adherence to and invasion of cultured human cells.
- Subjects :
- DNA, Bacterial
Mutant
Virulence
Microbiology
Bacterial Adhesion
Cell Line
law.invention
Plasmid
Bacterial Proteins
law
Heat shock protein
Genetics
Animals
Humans
HSP90 Heat-Shock Proteins
Molecular Biology
Porphyromonas gingivalis
Antiserum
Base Sequence
biology
biology.organism_classification
Antibodies, Bacterial
Molecular biology
Recombinant Proteins
Mutagenesis
Cell culture
Recombinant DNA
Rabbits
Plasmids
Subjects
Details
- ISSN :
- 15746968 and 03781097
- Volume :
- 225
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Letters
- Accession number :
- edsair.doi.dedup.....88530c9d6d44bde54b43b03f63539eef