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Drospirenone induces the accumulation of triacylglycerides in the fish hepatoma cell line, PLHC-1
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2019
- Publisher :
- Elsevier, 2019.
-
Abstract
- Drospirenone (DRO) is one of the most commonly used progestins reaching the aquatic environment through wastewater treatment plant effluents. It is a progesterone receptor agonist, and as such, can act primarily in the brain and reproductive organs of fish. In order to better understand and predict its effects, this work evaluates the lipidomic changes induced in PLHC-1 cells after exposure to drospirenone at concentrations below the EC10 (1 and 10 μM) by direct injection of the lipid extracts into a ESI(+/−) Orbitrap mass spectrometer. A significant accumulation of triacylglycerides, particularly long chain ones with unsaturated fatty acid moieties (TGs 46:2, 56:4–7; 58:5–8) and a concomitant decrease of diacylglycerides (DGs 32:1, 34:1–2, 36:1–2, 38:2–4) was observed after 48 h exposure to 10 μM DRO, which corresponded to an intracellular concentration of 8.3 ng·mg−1 protein. No significant alteration of PLHC-1 cell lipids was observed following exposure to 1 μM DRO. EC50 for the cytotoxicity of DRO ranged from 105 to 119 μM (24 h exposure) to 51–58 μM (48 h exposure). The study evidences a dysregulation of neutral lipid metabolism and increased TG/DG ratio in fish hepatic cells exposed to DRO.<br />Anna Marqueño acknowledges a pre-doctoral fellowship BES-2015-074842. Work financed by Ministerio de Ciencia e Innovación, under the project CGL2014-52144-P.
- Subjects :
- medicine.medical_specialty
Environmental Engineering
010504 meteorology & atmospheric sciences
010501 environmental sciences
01 natural sciences
Glycerides
Cyprinodontiformes
Drospirenone
Cell Line, Tumor
Internal medicine
Progesterone receptor
Lipidomics
medicine
Animals
Environmental Chemistry
Cytotoxicity
Waste Management and Disposal
Unsaturated fatty acid
Mineralocorticoid Receptor Antagonists
0105 earth and related environmental sciences
EC50
Mass spectrometry
Chemistry
PLHC-1 cells
fungi
Pollution
Endocrinology
Hepatocytes
Hepatic stellate cell
Androstenes
Water Pollutants, Chemical
Intracellular
medicine.drug
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Accession number :
- edsair.doi.dedup.....884d2d50f13bc75110c766f0046c039b