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Abundance of the Quorum-Sensing Factor Ax21 in Four Strains of Stenotrophomonas maltophilia Correlates with Mortality Rate in a New Zebrafish Model of Infection
- Source :
- PLoS ONE, Vol 8, Iss 6, p e67207 (2013), Dipòsit Digital de Documents de la UAB, Universitat Autònoma de Barcelona, PLoS ONE, CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET, PLoS ONE; Vol 8, SEDICI (UNLP), Universidad Nacional de La Plata, instacron:UNLP
- Publication Year :
- 2013
-
Abstract
- Stenotrophomonas maltophilia is a Gram-negative pathogen with emerging nosocomial incidence. Little is known about its pathogenesis and the genomic diversity exhibited by clinical isolates complicates the study of pathogenicity and virulence factors. Here, we present a strategy to identify such factors in new clinical isolates of S. maltophilia, incorporating an adultzebrafish model of S. maltophilia infection to evaluate relative virulence coupled to 2D difference gel electrophoresis to explore underlying differences in protein expression. In this study we report upon three recent clinical isolates and use the collection strain ATCC13637 as a reference. The adult-zebrafish model shows discrimination capacity, i.e. from very low to very high mortality rates, with clinical symptoms very similar to those observed in natural S. maltophilia infections in fish. Strain virulence correlates with resistance to human serum, in agreement with previous studies in mouse and rat and therefore supporting zebrafish as a replacement model. Despite its clinical origin, the collection strain ATCC13637 showed obvious signs of attenuation in zebrafish, with null mortality. Multilocus-sequence-typing analysis revealed that the most virulent strains, UV74 and M30, exhibit the strongest genetic similitude. Differential proteomic analysis led to the identification of 38 proteins with significantly different abundance in the three clinical strains relative to the reference strain. Orthologs of several of these proteins have been already reported to have a role in pathogenesis, virulence or resistance mechanisms thus supporting our strategy. Proof of concept is further provided by protein Ax21, whose abundance is shown here to be directly proportional to mortality in the zebrafish infection model. Indeed, recent studies have demonstrated that this protein is a quorum-sensing-related virulence factor.<br />Instituto de Biotecnologia y Biologia Molecular
- Subjects :
- Proteomics
Biología
Stenotrophomonas maltophilia
lcsh:Medicine
Pathogenesis
Virulence factor
Bacterial Adhesion
purl.org/becyt/ford/1 [https]
Gram Negative
lcsh:Science
Pathogen
Zebrafish
0303 health sciences
Multidisciplinary
Spectrometric Identification of Proteins
Virulence
Quorum Sensing
Animal Models
Bioquímica y Biología Molecular
Anti-Bacterial Agents
Bacterial Pathogens
Host-Pathogen Interaction
Stenotrophomonas
Phenotype
Proteomica
CIENCIAS NATURALES Y EXACTAS
Research Article
Oportunista
Difference gel electrophoresis
Biology
Microbiology
Multiresistencia
Ciencias Biológicas
03 medical and health sciences
Model Organisms
Bacterial Proteins
Drug Resistance, Bacterial
Animals
Humans
purl.org/becyt/ford/1.6 [https]
030304 developmental biology
030306 microbiology
lcsh:R
Bacteriology
biology.organism_classification
Quorum sensing
Disease Models, Animal
Emerging Infectious Diseases
Biofilms
lcsh:Q
Bacterial pathogens
Gram-Negative Bacterial Infections
Bacterial Biofilms
Protein Abundance
HeLa Cells
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, Vol 8, Iss 6, p e67207 (2013), Dipòsit Digital de Documents de la UAB, Universitat Autònoma de Barcelona, PLoS ONE, CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET, PLoS ONE; Vol 8, SEDICI (UNLP), Universidad Nacional de La Plata, instacron:UNLP
- Accession number :
- edsair.doi.dedup.....883827c6e694a91e04a0cccac59ba96e