Additional file 1: Table S1. of Vaccaria hypaphorine alleviates lipopolysaccharide-induced inflammation via inactivation of NFκB and ERK pathways in Raw 264.7 cells

Primer and its parameters of RT-PCR analysis. Table S2. Effects of Vaccaria hypaphorine on survival rate of different cell lines. Figure S1. Effects of different doses of Vaccaria Hypaphorine (12.5, 25 and 50 μM for 24 h) on the cell survival rate in response to LPS-stimulated RAW264.7 cells in vitro. (A), effects of pretreatment of different concentrations of Vaccaria Hypaphorine (12.5, 25 and 50 μM), Dex (100 μM) and Asp (1 mM) on cell viability of LPS (1 μg/ml)-challenged RAW264.7 cells determined with SRB assay. Values are mean ± S.D. LPS, lipopolysaccharide; Dex, Dexamethasone; Asp, Aspirin; Sulforhodamine B, SRB. Figure S2. Schematic indicating the inhibition of vaccaria hypaphorine to alleviate inflammation response by LPS in RAW264.7 cells. (DOCX 391 kb)