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Sea Cucumber Saponins Derivatives Alleviate Hepatic Lipid Accumulation Effectively in Fatty Acids-Induced HepG2 Cells and Orotic Acid-Induced Rats
- Source :
- Marine Drugs; Volume 20; Issue 11; Pages: 703
- Publication Year :
- 2022
-
Abstract
- The sulfated echinoside A (EA) and holothurin A (HA) are two prominent saponins in sea cucumber with high hemolytic activity but also superior lipid-lowering activity. Deglycosylated derivatives EA2 and HA2 exhibit low hemolysis compared to EA and HA, but their efficacies on lipid metabolism regulation remains unknown. In this study, fatty acids-treated HepG2 cells and orotic acid-treated rats were used to investigate the lipid-lowering effects of sea cucumber saponin derivatives. Both the saponin and derivatives could effectively alleviate lipid accumulation in HepG2 model, especially EA and EA2. Moreover, though the lipid-lowering effect of EA2 was not equal with EA at the same dosage of 0.05% in diet, 0.15% dosage of EA2 significantly reduced hepatic steatosis rate, liver TC and TG contents by 76%, 41.5%, and 63.7%, respectively, compared to control and reversed liver histopathological features to normal degree according to H&E stained sections. Possible mechanisms mainly included enhancement of fatty acids β-oxidation and cholesterol catabolism through bile acids synthesis and excretion, suppression of lipogenesis and cholesterol uptake. It revealed that the efficacy of EA2 on lipid metabolism regulation was dose-dependent, and 0.15% dosage of EA2 possessed better efficacy with lower toxicity compared to 0.05% dosage of EA.
- Subjects :
- Orotic Acid
saponins
derivatives
cholesterol metabolism
lipogenesis
fatty acids β-oxidation
Sea Cucumbers
Fatty Acids
Pharmaceutical Science
Hep G2 Cells
Saponins
Lipid Metabolism
Rats
Cholesterol
Liver
Non-alcoholic Fatty Liver Disease
Drug Discovery
Animals
Humans
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Subjects
Details
- ISSN :
- 16603397
- Volume :
- 20
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Marine drugs
- Accession number :
- edsair.doi.dedup.....8833b3ec221586bcd2cda86da7fc3f74