Supplementary Figure 4 from The Chemokine (CCL2–CCR2) Signaling Axis Mediates Perineural Invasion

A. Close up views of the photomicrographs from Figure 4 show an association between the PC-3 cells and the wild type DRG neurites (Figure 4A), but fewer associations between the PC-3 cells and CCL2 -/- DRG neurites (Figure 4B). B. DRG's were harvested from wild type and CCL2 -/- mice and explanted in 15µl of growth-factor reduced Matrigel. Photographs were taken every other day. Neurite outgrowth rate was measured by measuring the area covered by the neurites and subtracting the center of the DRG (MetaMorph, Molecular Devices, Sunnyvale, CA).