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High-Throughput Screening of an Octanoic Acid Producer Strain Library Enables Detection of New Targets for Increasing Titers in Saccharomyces cerevisiae
- Source :
- ACS Synthetic Biology
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- Octanoic acid is an industrially relevant compound with applications in antimicrobials or as a precursor for biofuels. Microbial biosynthesis through yeast is a promising alternative to current unsustainable production methods. To increase octanoic acid titers in Saccharomyces cerevisiae, we use a previously developed biosensor that is based on the octanoic acid responsive pPDR12 promotor coupled to GFP. We establish a biosensor strain amenable for high-throughput screening of an octanoic acid producer strain library. Through development, optimization, and execution of a high-throughput screening approach, we were able to detect two new genetic targets, KCS1 and FSH2, which increased octanoic acid titers through combined overexpression by about 55% compared to the parental strain. Neither target has yet been reported to be involved in fatty acid biosynthesis. The presented methodology can be employed to screen any genetic library and thereby more genes involved in improving octanoic acid production can be detected in the future.
- Subjects :
- 0106 biological sciences
0303 health sciences
Strain (chemistry)
biology
High-throughput screening
Saccharomyces cerevisiae
Biomedical Engineering
General Medicine
biology.organism_classification
01 natural sciences
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Yeast
Green fluorescent protein
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
chemistry
Biochemistry
010608 biotechnology
Genetic library
Gene
030304 developmental biology
Subjects
Details
- ISSN :
- 21615063
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- ACS Synthetic Biology
- Accession number :
- edsair.doi.dedup.....88305193568d09616e08998b5e10dc21