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Godzilla-dependent transcytosis promotes Wingless signalling in Drosophila wing imaginal discs
- Source :
- Nature cell biology
- Publication Year :
- 2016
-
Abstract
- The apical and basolateral membranes of epithelia are insulated from each other, preventing the transfer of extracellular proteins from one side to the other1. Thus, a signalling protein produced apically is not expected to reach basolateral receptors. Evidence suggests that Wingless, the main Drosophila Wnt is secreted apically in the embryonic epidermis2, 3. However, in the wing imaginal disc epithelium, Wingless is mostly seen on the basolateral membrane where it spreads from secreting to receiving cells 4, 5. Here we examine the apico-basal movement of Wingless in Wingless-producing cells of wing imaginal discs. We find that it is presented first on the apical surface before making its way to the basolateral surface, where it is released and allowed to interact with signalling receptors. We show that Wingless transcytosis involves Dynamin-dependent endocytosis from the apical surface. Subsequent trafficking from early apical endosomes to the basolateral surface requires Godzilla, a member of the RNF family of membrane-anchored E3 ubiquitin ligases. Without such transport, Wingless signalling is strongly reduced in this tissue.
- Subjects :
- 0301 basic medicine
animal structures
Endosome
Ubiquitin-Protein Ligases
Green Fluorescent Proteins
Signalling
Endosomes
Wnt1 Protein
Biology
Endocytosis
Exocytosis
Article
Animals, Genetically Modified
03 medical and health sciences
Wnt
0302 clinical medicine
Animals
Drosophila Proteins
Wings, Animal
In Situ Hybridization, Fluorescence
Epithelial polarity
Microscopy, Confocal
integumentary system
fungi
Wnt signaling pathway
Cell Biology
3. Good health
Cell biology
Imaginal disc
Ubiquitin ligase
030104 developmental biology
Drosophila melanogaster
Transcytosis
Imaginal Discs
RNA Interference
Epithelia
030217 neurology & neurosurgery
Drosophila Protein
Intracellular trafficking
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 14764679 and 14657392
- Volume :
- 18
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Nature cell biology
- Accession number :
- edsair.doi.dedup.....881593ab88c47ded223cae38d5397391