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Air-Drying Beds Reduce the Quantities of Antibiotic Resistance Genes and Class 1 Integrons in Residual Municipal Wastewater Solids
- Source :
- Environmental Science & Technology. 47:9965-9971
- Publication Year :
- 2013
- Publisher :
- American Chemical Society (ACS), 2013.
-
Abstract
- This study investigated whether air-drying beds reduce antibiotic resistance gene (ARG) concentrations in residual municipal wastewater solids. Three laboratory-scale drying beds were operated for a period of nearly 100 days. Real-time PCR was used to quantify 16S rRNA genes, 16S rRNA genes specific to fecal bacteria (AllBac) and human fecal bacteria (HF183), the integrase gene of class 1 integrons (intI1), and five ARGs representing a cross-section of antibiotic classes and resistance mechanisms (erm(B), sul1, tet(A), tet(W), and tet(X)). Air-drying beds were capable of reducing all gene target concentrations by 1 to 5 orders of magnitude, and the nature of this reduction was consistent with both a net decrease in the number of bacterial cells and a lack of selection within the microbial community. Half-lives varied between 1.5 d (HF183) and 5.4 d (tet(X)) during the first 20 d of treatment. After the first 20 d of treatment, however, half-lives varied between 8.6 d (tet(X)) and 19.3 d (AllBac), and 16S rRNA gene, intI1, and sul1 concentrations did not change (P0.05). These results demonstrate that air-drying beds can reduce ARG and intI1 concentrations in residual municipal wastewater solids within timeframes typical of operating practices.
- Subjects :
- Bacteria
Waste management
Drug Resistance, Microbial
General Chemistry
Wastewater
Real-Time Polymerase Chain Reaction
Residual
Waste Disposal, Fluid
Anti-Bacterial Agents
Integrons
Bacterial Proteins
RNA, Ribosomal, 16S
Water Pollution, Chemical
Environmental Chemistry
Environmental science
Air drying
Water Pollutants, Chemical
Antibiotic resistance genes
Subjects
Details
- ISSN :
- 15205851 and 0013936X
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- Environmental Science & Technology
- Accession number :
- edsair.doi.dedup.....88126bf3c02abf6809dd8ec73e7bb5ce