Immobilization of lipases on lignocellulosic bamboo powder for biocatalytic transformations in batch and continuous flow

Lignocellulose-based bamboo powder was used as pristine support to immobilize two different biocatalysts: Candida Antarctica lipase B and Rhizomucor miehei lipase. Both, IB-CalB and IB-RM, physically absorbed on bamboo carrier presented considerable recycling efficiency, up to 10 consecutive cycles, in batch mode for the ethyl palmitate synthesis with conversion about 50 and 80 %, respectively. The kinetic resolution of racemic-1-phenylethanol mediated by IB-CalB showed a higher conversion against the IB-RM in batch mode. IB-CalB packed into a glass column reactor was used to carry on a kinetic resolution in flow mode regime with an intensification conversion up to 45 % with 99 % e.e. The easy and low-cost method of the lipase adsorption without pre-treatment of the carrier can be promising for industrial biocatalytic transformation and immobilization strategy.