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Bronchoalveolar lavage quality influences the T4/T8 ratio in sarcoidosis
- Source :
- Respiratory Medicine. 101(9):2025-2030
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- Summary Background Sarcoidosis is characterised by a T-lymphocytic alveolitis with a typically increased T4/T8 ratio. The diagnostic value of this ratio is under debate. Aim of the work We prospectively evaluated the influence of BAL pre-lavage and the impact of bronchial contamination on BAL differential cell count in 108 BAL specimens obtained from patients with histologically confirmed sarcoidosis. Methods BAL was performed by instilling 150–300ml normal saline either in the middle lobe or the lingula. Fifty-one patients (47%) underwent additional pre-lavage with 50ml normal saline. Bronchial contamination was assessed by semi-quantitative analysis of mucus, ciliated and squamous cells in the untreated BAL recovery. Results Pre-lavage did neither influence the lavage cellularity nor extend of contamination of the BAL. Content of mucus and ciliated cells, indicating bronchial contamination, showed a high correlation (Kendal's tau=0.61). Presence of either mucus or ciliated cells in the BAL recovery was associated with a significant lower T4/T8 ratio (mucus: 4.9 vs. 8.0, p =0.009; ciliated cells: 4.1vs.7.4, p =0.001). Squamous cells in the BAL recovery representing oropharyngeal contamination did not significantly influence the T4/T8 ratio (7.7 vs. 5.6, p =0.10). Conclusion Bronchial contamination of BAL as determined by the presence of mucus and ciliated cells in the recovery decreases the T4/T8 ratio of BAL in sarcoidosis.
- Subjects :
- Male
Pulmonary and Respiratory Medicine
Systemic disease
Pathology
medicine.medical_specialty
Sarcoidosis
medicine.medical_treatment
CD4-CD8 Ratio
Cell Count
Sodium Chloride
Bronchoalveolar Lavage
Specimen Handling
Sarcoidosis, Pulmonary
T-Lymphocyte Subsets
Medicine
Humans
Lavage quality
Pulmonary pathology
Cilia
Lymphocyte Count
Prospective Studies
Saline
BAL
medicine.diagnostic_test
business.industry
Middle Lobe
respiratory system
medicine.disease
Mucus
respiratory tract diseases
Bronchoalveolar lavage
Female
business
Bronchoalveolar Lavage Fluid
T4/T8 ratio
Subjects
Details
- ISSN :
- 09546111
- Volume :
- 101
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Respiratory Medicine
- Accession number :
- edsair.doi.dedup.....87f349b6f15077e2d718a69659827e93
- Full Text :
- https://doi.org/10.1016/j.rmed.2006.11.027