Back to Search
Start Over
A General Process-Based Model for Describing the Metabolic Shift in Microbial Cell Cultures
- Source :
- Frontiers in Microbiology, Frontiers in Microbiology, Vol 11 (2020)
- Publication Year :
- 2020
- Publisher :
- Frontiers Media SA, 2020.
-
Abstract
- The metabolic shift between respiration and fermentation at high glucose concentration is a widespread phenomenon in microbial world, and it is relevant for the biotechnological exploitation of microbial cell factories, affecting the achievement of high-cell-densities in bioreactors. Starting from a model already developed for the yeast Saccharomyces cerevisiae, based on the System Dynamics approach, a general process-based model for two prokaryotic species of biotechnological interest, such as Escherichia coli and Bacillus subtilis, is proposed. The model is based on the main assumption that glycolytic intermediates act as central catabolic hub regulating the shift between respiratory and fermentative pathways. Furthermore, the description of a mixed fermentation with secondary by-products, characteristic of bacterial metabolism, is explicitly considered. The model also represents the inhibitory effect on growth and metabolism of self-produced toxic compounds relevant in assessing the late phases of high-cell density culture. Model simulations reproduced data from experiments reported in the literature with different strains of non-recombinant and recombinant E. coli and B. subtilis cultured in both batch and fed-batch reactors. The proposed model, based on simple biological assumptions, is able to describe the main dynamics of two microbial species of relevant biotechnological interest. It demonstrates that a reductionist System Dynamics approach to formulate simplified macro-kinetic models can provide a robust representation of cell growth and accumulation in the medium of fermentation by-products.
- Subjects :
- Crabtree/Warburg effect
Microbiology (medical)
Saccharomyces cerevisiae
lcsh:QR1-502
Microbial metabolism
Bacillus subtilis
medicine.disease_cause
Microbiology
lcsh:Microbiology
03 medical and health sciences
self-inhibition
System Dynamics (SD) model
Escherichia coli
Bioreactor
medicine
Bacillus subtili
overflow metabolism
Overflow metabolism
Original Research
030304 developmental biology
0303 health sciences
biology
030306 microbiology
Chemistry
biology.organism_classification
high cell-density culture
Yeast
Fermentation
Biochemical engineering
Subjects
Details
- ISSN :
- 1664302X
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Frontiers in Microbiology
- Accession number :
- edsair.doi.dedup.....87ea325bf133cfdfcf1bb920413076ab