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Genetic Diversity of Purple Passion Fruit, Passiflora edulis f. edulis, Based on Single-Nucleotide Polymorphism Markers Discovered through Genotyping by Sequencing
- Source :
- Diversity, Volume 13, Issue 4, Diversity, Vol 13, Iss 144, p 144 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Orphan crops, which include many of the tropical fruit species used in the juice industry, lack genomic resources and breeding efforts. Typical of this dilemma is the lack of commercial cultivars of purple passion fruit, Passiflora edulis f. edulis, and of information on the genetic resources of its substantial semiwild gene pool. In this study, we develop single-nucleotide polymorphism (SNP) markers for the species and show that the genetic diversity of this fruit crop has been reduced because of selection for cultivated genotypes compared to the semiwild landraces in its center of diversity. A specific objective of the present study was to determine the genetic diversity of cultivars, genebank accession, and landraces through genotyping by sequencing (GBS) and to conduct molecular evaluation of a broad collection for the species P. edulis from a source country, Colombia. We included control genotypes of yellow passion fruit, P. edulis f. flavicarpa. The goal was to evaluate differences between fruit types and compare landraces and genebank accessions from in situ accessions collected from farmers. In total, 3820 SNPs were identified as informative for this diversity study. However, the majority distinguished yellow and purple passion fruit, with 966 SNPs useful in purple passion fruits alone. In the population structure analysis, purple passion fruits were very distinct from the yellow ones. The results for purple passion fruits alone showed reduced diversity for the commercial cultivars while highlighting the higher diversity found among landraces from wild or semi-wild conditions. These landraces had higher heterozygosity, polymorphism, and overall genetic diversity. The implications for genetics and breeding as well as evolution and ecology of purple passion fruits based on the extant landrace diversity are discussed with consideration of manual or pollinator-assisted hybridization of this species.
- Subjects :
- 0106 biological sciences
Genotyping by sequencing
Single-nucleotide polymorphism
01 natural sciences
SNP markers
Passiflora
Crop
GBS technique
03 medical and health sciences
Genotype
Cultivar
lcsh:QH301-705.5
030304 developmental biology
Nature and Landscape Conservation
0303 health sciences
Genetic diversity
Ecology
biology
Ecological Modeling
food and beverages
population structure
genetic diversity
biology.organism_classification
Agricultural and Biological Sciences (miscellaneous)
humanities
Horticulture
lcsh:Biology (General)
Gene pool
human activities
ward-MLM
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 14242818
- Database :
- OpenAIRE
- Journal :
- Diversity
- Accession number :
- edsair.doi.dedup.....87d903d42db6042cf740c33c722f46d6
- Full Text :
- https://doi.org/10.3390/d13040144