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Phenotype and Hierarchy of Two Transgenic T Cell Lines Targeting the Respiratory Syncytial Virus KdM282-90 Epitope Is Transfer Dose-Dependent
- Source :
- PLoS ONE, Vol 11, Iss 1, p e0146781 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- In this study, we compared two lines of transgenic CD8+ T cells specific for the same KdM282-90 epitope of respiratory syncytial virus in the CB6F1 hybrid mouse model. Here we found that these two transgenic lines had similar in vivo abilities to control viral load after respiratory syncytial virus infection using adoptive transfer. Transfer of the TRBV13-2 line resulted in higher levels of IL-6 and MIP1-α in the lung than TRBV13-1 transfer. Interestingly, when large numbers of cells were co-transferred, the lines formed a hierarchy, with TRBV13-2 being immunodominant over TRBV13-1 in the mediastinal lymph node despite no identifiable difference in proliferation or apoptosis between the lines. This hierarchy was not established when lower cell numbers were transferred. The phenotype and frequency of proliferating cells were also cell transfer dose-dependent with higher percentages of CD127loCD62LloKLRG1lo and proliferating cells present when lower numbers of cells were transferred. These results illustrate the importance of cell number in adoptive transfer experiments and its influence on the phenotype and hierarchy of the subsequent T cell response.
- Subjects :
- 0301 basic medicine
Male
Adoptive cell transfer
T cell
Cell
Epitopes, T-Lymphocyte
lcsh:Medicine
Apoptosis
Mice, Transgenic
Respiratory Syncytial Virus Infections
Biology
CD8-Positive T-Lymphocytes
Virus
Epitope
Interleukin-7 Receptor alpha Subunit
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Cytotoxic T cell
Animals
Lectins, C-Type
L-Selectin
Receptors, Immunologic
lcsh:Science
Lung
Cell Proliferation
Chemokine CCL3
Mice, Inbred BALB C
Multidisciplinary
Cell growth
Interleukin-6
lcsh:R
Mediastinum
Virology
Adoptive Transfer
Cell biology
Respiratory Syncytial Viruses
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Phenotype
Female
lcsh:Q
Lymph Nodes
CD8
030215 immunology
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....87d50a2683beaff0cfc1e9c624677c52