Back to Search
Start Over
Rapid Expansion of Virus-Specific CD4+ T Cell Types in the CNS of Susceptible Mice Infected with Theiler’s Virus
- Source :
- International Journal of Molecular Sciences, Vol 21, Iss 7719, p 7719 (2020), International Journal of Molecular Sciences, Volume 21, Issue 20
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- The infection of susceptible mice with Theiler&rsquo<br />s murine encephalomyelitis virus (TMEV) induces a T cell-mediated demyelinating disease. This system has been studied as a relevant infection model for multiple sclerosis (MS). Therefore, defining the type of T cell responses and their functions is critically important for understanding the relevant pathogenic mechanisms. In this study, we adoptively transferred naive VP2-specific TCR-Tg CD4+ T cells into syngeneic susceptible SJL mice and monitored the development of the disease and the activation and proliferation of CD4+ T cells during the early stages of viral infection. The preexisting VP2-specific naive CD4+ T cells promoted the pathogenesis of the disease in a dose-dependent manner. The transferred VP2-specific CD4+ T cells proliferated rapidly in the CNS starting at 2&ndash<br />3 dpi. High levels of FoxP3+CD4+ T cells were found in the CNS early in viral infection (3 dpi) and persisted throughout the infection. Activated VP2-specific FoxP3+CD4+ T cells inhibited the production of IFN-&gamma<br />but not IL-17, via the same VP2-specific CD4+ T cells without interfering in proliferation. Thus, the early presence of regulatory T cells in the CNS with viral infection may favor the induction of pathogenic Th17 cells over protective Th1 cells in susceptible mice, thereby establishing the pathogenesis of virus-induced demyelinating disease.
- Subjects :
- 0301 basic medicine
viruses
T cell
Inflammation
virus
Disease
Biology
Catalysis
Virus
lcsh:Chemistry
Inorganic Chemistry
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Th cells
medicine
Demyelinating disease
Physical and Theoretical Chemistry
FoxP3+CD4+ T cells
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Multiple sclerosis
Organic Chemistry
virus diseases
FOXP3
General Medicine
biochemical phenomena, metabolism, and nutrition
medicine.disease
Virology
nervous system diseases
Computer Science Applications
030104 developmental biology
medicine.anatomical_structure
nervous system
lcsh:Biology (General)
lcsh:QD1-999
inflammation
demyelination
medicine.symptom
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....87cedb3ac38ec692f66dc9c68c934997