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Evaluation of immuno-efficacy of a novel DNA vaccine encoding Toxoplasma gondiirhoptry protein 38 (TgROP38) against chronic toxoplasmosis in a murine model
- Source :
- BMC Infectious Diseases
- Publication Year :
- 2014
- Publisher :
- BioMed Central, 2014.
-
Abstract
- Background Toxoplasma gondii is an obligate intracellular parasite which can infect almost all mammalian animals, leading to toxoplasmosis. T. gondii rhoptry protein 38 (TgROP38) is an active rhoptry protein kinase which is involved in the inhibitory effect on host cell transcription by down-regulating the MAPK signaling track. Methods TgROP38 gene was amplified and inserted into eukaryotic vector pVAX I and formed the DNA vaccine pVAX-ROP38. Mice in the experimental group were intramuscularly immunized with pVAX-ROP38 and those injected with pVAX I, PBS or nothing were treated as controls. After three injections at two week intervals, all mouse groups were challenged intraperitoneally with 1000 tachyzoites of the virulent T. gondii RH strain (Type I, ToxoDB #10) and 10 cysts of the PRU strain (Type II, ToxoDB #1), respectively. Results Mice inoculated with pVAX-ROP38 vaccine had a higher level of IgG antibodies (P
- Subjects :
- DNA vaccine
Protozoan Vaccines
Mouse
Protective immunity
Protozoan Proteins
Toxoplasma gondii
Antibodies, Protozoan
Injections, Intramuscular
Microbiology
DNA vaccination
Mice
Immune system
Immunity
medicine
Vaccines, DNA
Animals
Humans
Cell Proliferation
Immunity, Cellular
Rhoptry
biology
Vaccination
Protein-Tyrosine Kinases
biology.organism_classification
medicine.disease
Virology
Toxoplasmosis
Disease Models, Animal
Infectious Diseases
HEK293 Cells
Toxoplasmosis, Animal
TgROP38
Immunoglobulin G
Interleukin-2
Female
Toxoplasma
Lymphoproliferative response
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712334
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- BMC Infectious Diseases
- Accession number :
- edsair.doi.dedup.....87cc0374ae3eea3d31f8b72e4f91373a