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In vitro toxicological assessment of flumethrin’s effects on MCF-7 breast cancer cells
- Source :
- Human & Experimental Toxicology. 40:2165-2177
- Publication Year :
- 2021
- Publisher :
- SAGE Publications, 2021.
-
Abstract
- Pyrethroid pesticides are frequently used for household insect control of insects and in agriculture and livestock. Flumethrin is a pyrethroid that is used against ectoparasites in many animals. The goal of this study was to evaluate the cytotoxic, apoptotic, genotoxic, and estrogenic effects of flumethrin on the mammalian breast cancer cell line (MCF-7). Compared with control groups, a dose-dependent decrease was observed in cell viability at concentrations of 100 µM and higher. The cytotoxic and apoptotic effects detected by LDH assay and AO/EtBr staining increased significantly at a concentration of 1000 µM. The expression of BCL2, which is an anti-apoptotic gene, significantly decreased, whereas BAX, TP53, and P21 expression significantly increased. The results of a comet assay indicated that flumethrin significantly changed tail length, tail % DNA, tail moment, and Olive tail moment in concentrations above 1 and 10 µM. In addition, a 0.1 µM concentration of flumethrin affected ERα receptor mediated cell proliferation and increased transcription of estrogen-responsive pS2 (TFF1) and progesterone receptor (PGR) genes. As a result, flumethrin-induced apoptosis and cytotoxicity at a high concentration, while induced genotoxicity even at lower concentrations. Flumethrin is an endocrine disrupting insecticide with estrogenic effects at very low concentrations.
- Subjects :
- Cyclin-Dependent Kinase Inhibitor p21
0301 basic medicine
Cell Survival
Health, Toxicology and Mutagenesis
Apoptosis
Breast Neoplasms
Endocrine Disruptors
010501 environmental sciences
Toxicology
medicine.disease_cause
Flumethrin
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Pyrethrins
Progesterone receptor
medicine
Estrogen Receptor beta
Humans
Cytotoxicity
0105 earth and related environmental sciences
Chemistry
Cell growth
Estrogen Receptor alpha
Estrogens
General Medicine
Molecular biology
Gene Expression Regulation, Neoplastic
Comet assay
030104 developmental biology
Proto-Oncogene Proteins c-bcl-2
MCF-7
MCF-7 Cells
Female
Trefoil Factor-1
Tumor Suppressor Protein p53
Genotoxicity
DNA Damage
Mutagens
Subjects
Details
- ISSN :
- 14770903 and 09603271
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Human & Experimental Toxicology
- Accession number :
- edsair.doi.dedup.....87b1af1f005f07027c044c2afeb67e86