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Preliminary characterization of a soluble immunosuppressive molecule from DBA/2 spleen cells using monoclonal antibody immunoadsorbence
- Source :
- Cellular Immunology. 90:303-313
- Publication Year :
- 1985
- Publisher :
- Elsevier BV, 1985.
-
Abstract
- In a previous publication a monoclonal antibody (B16G) which appeared to recognize T suppressor cells and a T-suppressor factor (TsF) in the spleens of DBA/2 mice was described. B16G appears to be directed to a public specificity of DBA/2 TsF and therefore has been shown to inhibit a variety of immunological reactions. The present study involves preliminary characterization of the material with which B16G reacts. It was found that the B16G-reactive protein (putative TsF) could be absorbed and eluted specifically from a B16G immunoadsorbent column. Material eluting from the B16G column reacted with B16G in an ELISA and appeared to run as two or more bands of 40–45 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The eluted material was biologically active (i.e., suppressive) in the standard assay (mixed leukocyte reaction of DBA/2 splenocytes with B10.BR targets), and its suppressive activity was abrogated by the addition of B16G to the mixed leukocyte reaction cultures. Sephadex G-150 chromatography of the B16G-reactive material showed that under these conditions, its native molecular mass was between 80–90 kDa, indicating that it might occur as a dimer under natural conditions.
- Subjects :
- medicine.drug_class
Immunology
Enzyme-Linked Immunosorbent Assay
Spleen
Biology
Monoclonal antibody
T-Lymphocytes, Regulatory
Chromatography, Affinity
Mice
Immune system
Suppressor Factors, Immunologic
medicine
Splenocyte
Animals
Antigens
Immunosorbent Techniques
Gel electrophoresis
Lymphokines
Molecular mass
Antibodies, Monoclonal
Biological activity
Molecular biology
medicine.anatomical_structure
Mice, Inbred DBA
Sephadex
Lymphocyte Culture Test, Mixed
Subjects
Details
- ISSN :
- 00088749
- Volume :
- 90
- Database :
- OpenAIRE
- Journal :
- Cellular Immunology
- Accession number :
- edsair.doi.dedup.....879e04d63fc0ee7ac9840eeaec659835