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Growth and expression of relevant metabolic genes of Clostridium thermocellum cultured on lignocellulosic residues
- Source :
- Journal of Industrial Microbiology and Biotechnology. 44:825-834
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- The plant cell wall is a source of fermentable sugars in second-generation bioethanol production. However, cellulosic biomass hydrolysis remains an obstacle to bioethanol production in an efficient and low-cost process. Clostridium thermocellum has been studied as a model organism able to produce enzymatic blends that efficiently degrade lignocellulosic biomass, and also as a fermentative microorganism in a consolidated process for the conversion of lignocellulose to bioethanol. In this study, a C. thermocellum strain (designated B8) isolated from goat rumen was characterized for its ability to grow on sugarcane straw and cotton waste, and to produce cellulosomes. We also evaluated C. thermocellum gene expression control in the presence of complex lignocellulosic biomasses. This isolate is capable of growing in the presence of microcrystalline cellulose, sugarcane straw and cotton waste as carbon sources, producing free enzymes and residual substrate-bound proteins (RSBP). The highest growth rate and cellulase/xylanase production were detected at pH 7.0 and 60 °C, after 48 h. Moreover, this strain showed different expression levels of transcripts encoding cellulosomal proteins and proteins with a role in fermentation and catabolic repression.
- Subjects :
- 0301 basic medicine
Lignocellulosic biomass
Bioengineering
Cellulosomes
Cellulase
Lignin
Applied Microbiology and Biotechnology
Microbiology
Clostridium thermocellum
03 medical and health sciences
Animals
Biomass
Food science
biology
Chemistry
Goats
food and beverages
Gene Expression Regulation, Bacterial
biology.organism_classification
Xylosidases
030104 developmental biology
Cellulosic ethanol
Biofuel
Fermentation
biology.protein
Xylanase
Biotechnology
Subjects
Details
- ISSN :
- 14765535 and 13675435
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Journal of Industrial Microbiology and Biotechnology
- Accession number :
- edsair.doi.dedup.....878b06414a27d5d6d3e2fad1056a79ea