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Quantification of endogenous aromatic cytokinins in Pinus radiata embryonal masses after application of heat stress during initiation of somatic embryogenesis
- Source :
- Trees. 35:1075-1080
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Short-term exposure to high temperatures modulates the aromatic cytokinin profile of radiata pine embryonal masses when measured 8 weeks after initiation. Abstract Recent research has demonstrated that cytokinins could be involved in abiotic stress responses. However, little research has been published about the role of endogenous aromatic cytokinins during these processes. Therefore, in this study, different temperature and incubation periods (23 oC, 8 weeks; 40 oC, 4 h; 50 oC, 30 min; 60 oC, 5 min) were applied during induction of radiata pine somatic embryogenesis and the aromatic cytokinin content of the resulting embryonal masses was measured. Results indicated that temperature during induction had a significant effect on the profile of most of the aromatic cytokinins detected. All tested higher temperatures than control provoked a decline in some free bases, including N6-benzyladenine, ortho-Topolin and para-Topolin. On the other hand, the values of N6-benzyladenine nucleotides, ribosides and irreversible metabolites (9G), as well as para-Topolin ribosides, increased in embryonal masses originating from 40 oC treatment. These results suggest that despite not being traditionally considered part of the stress response machinery, aromatic cytokinins might be involved in heat-stress responses during early stages of somatic embryogenesis in radiata pine.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Somatic embryogenesis
Physiology
Radiata
Endogeny
Plant Science
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Incubation
Embryogenic tissue
N6-Benzyladenine
Orto-topolin
Para-topolin
Radiata pine
Temperature
Ecology
biology
Chemistry
Abiotic stress
Pinus radiata
fungi
food and beverages
Plant physiology
Forestry
biology.organism_classification
030104 developmental biology
Biochemistry
Cytokinin
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14322285 and 09311890
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Trees
- Accession number :
- edsair.doi.dedup.....87867b2e31280d77e13588c51af0cbda