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Electrochemical Interrogation of DNA Monolayers on Gold Surfaces

Authors :
Lihua Wang
Chunhai Fan
Lin He
Haiping Wu
Shiping Song
Ruojun Lao
Zhizhou Zhang
Source :
Analytical Chemistry. 77:6475-6480
Publication Year :
2005
Publisher :
American Chemical Society (ACS), 2005.

Abstract

In this report, we systematically investigated DNA immobilization at gold surfaces with electrochemical techniques. Comparative cyclic voltammetric and chronocoulometric studies suggested that DNA monolayers immobilized at gold surfaces were not homogeneous. Nonspecific Au-DNA interactions existed even with the treatment of mercaptohexanol, which was known to competitively remove loosely bound DNA at gold surfaces. While both thiolated and nonthiolated DNA formed monolayers on gold surfaces, their hybridization abilities were distinctly different. In contrast to thiolated DNA probes, nonthiolated DNA probes immobilized at gold surfaces were essentially nonhybridizable. The experimental results presented here might be useful for the design of high-performance electrochemical DNA sensors.

Details

ISSN :
15206882 and 00032700
Volume :
77
Database :
OpenAIRE
Journal :
Analytical Chemistry
Accession number :
edsair.doi.dedup.....877ed9956770fde8ada5938dafc9be7b