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Gene disruptions using P transposable elements: an integral component of the Drosophila genome project

Authors :
John Roote
Dianne M. Stern
Todd R. Laverty
Allan C. Spradling
Gerald M. Rubin
István Kiss
Source :
Proceedings of the National Academy of Sciences. 92:10824-10830
Publication Year :
1995
Publisher :
Proceedings of the National Academy of Sciences, 1995.

Abstract

Biologists require genetic as well as molecular tools to decipher genomic information and ultimately to understand gene function. The Berkeley Drosophila Genome Project is addressing these needs with a massive gene disruption project that uses individual, genetically engineered P transposable elements to target open reading frames throughout the Drosophila genome. DNA flanking the insertions is sequenced, thereby placing an extensive series of genetic markers on the physical genomic map and associating insertions with specific open reading frames and genes. Insertions from the collection now lie within or near most Drosophila genes, greatly reducing the time required to identify new mutations and analyze gene functions. Information revealed from these studies about P element site specificity is being used to target the remaining open reading frames.

Details

ISSN :
10916490 and 00278424
Volume :
92
Database :
OpenAIRE
Journal :
Proceedings of the National Academy of Sciences
Accession number :
edsair.doi.dedup.....876a9797c0b2a1b3631a352f8e5885a3
Full Text :
https://doi.org/10.1073/pnas.92.24.10824