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Lack of elevated pre-ART elastase-ANCA levels in patients developing TB-IRIS
- Source :
- PloS one, 15 (12 December, PLoS ONE, 15(12 December):e0244800. Public Library of Science, PLoS ONE, PLoS ONE, Vol 15, Iss 12, p e0244800 (2020)
- Publication Year :
- 2020
-
Abstract
- Background Tuberculosis-associated immune reconstitution inflammatory syndrome (TB-IRIS) in HIV-TB co-infected patients receiving antiretroviral therapy (ART) has been linked to neutrophil activation. Anti-neutrophil cytoplasmic antibodies (ANCAs) are also associated with neutrophil activation. Since ANCAs are reportedly skewed in TB and HIV infections, we investigated plasma levels of 7 ANCAs in TB-IRIS patients. Methods We retrospectively compared 17 HIV-TB patients who developed TB-IRIS with controls of similar CD4 count, age and gender who did not (HIV+TB+ n = 17), HIV-infected patients without TB (HIV+TB-, n = 17) and 10 HIV-negative (HIV-TB-) controls. Frozen plasma was collected before ART, at 3 and 9 months of ART, and examined by ELISA for levels of 7 ANCAs directed against; Proteinase 3 (PR3), Myeloperoxidase (MPO), Permeability-increasing protein (BPI), Elastase, Cathepsin, Lysozyme, and Lactoferrin. Results Compared to HIV+TB+ controls, pre-ART anti-elastase levels were lower in TB-IRIS patients (p = 0.026) and HIV-TB- controls (p = 0.044), whereas other ANCAs did not show significant differences between groups at any time point. A significant decrease over time could be observed in TB-IRIS patients during ART for anti -PR3 (p = 0.027), -lysozyme (p = 0.011), and -lactoferrin (p = 0.019). Conversely, HIV+TB+ controls showed a significant decrease over time for anti -MPO (p = 0.002), -lyzosyme (p = 0.002) and -elastase (p < 0.001). Conclusion The lack of elevated anti-elastase levels in TB-IRIS patients as opposed to HIV+TB+ controls correspond to previous findings of lowered immune capacity in patients that will develop TB-IRIS. This may suggest a specific role for anti-elastase, elastase or even matrix-metalloproteinases in TB-IRIS. The precise dynamics of neutrophil activation in HIV-TB merits further investigation and could provide more insight in the early mechanisms leading up to TB-IRIS.<br />SCOPUS: ar.j<br />info:eu-repo/semantics/published
- Subjects :
- 0301 basic medicine
Male
Bacterial Diseases
Neutrophils
Physiology
HIV Infections
Biochemistry
White Blood Cells
Medical Conditions
Proteinase 3
Immune Reconstitution Inflammatory Syndrome
Animal Cells
Immune Physiology
Blood plasma
Immunologie
Medicine and Health Sciences
Medicine
Public and Occupational Health
Immune Response
Multidisciplinary
Immune System Proteins
biology
Pancreatic Elastase
Lactoferrin
Elastase
Vaccination and Immunization
Body Fluids
Infectious Diseases
Blood
Anti-Retroviral Agents
Myeloperoxidase
Female
Antibody
Cellular Types
Anatomy
Engineering sciences. Technology
Research Article
Adult
Tuberculosis
Science
Immune Cells
030106 microbiology
Immunology
Antiretroviral Therapy
Blood Plasma
Antibodies
Antibodies, Antineutrophil Cytoplasmic
03 medical and health sciences
Signs and Symptoms
Immune reconstitution inflammatory syndrome
Antiviral Therapy
Humans
cardiovascular diseases
Inflammation
Blood Cells
business.industry
Biology and Life Sciences
Proteins
Cell Biology
medicine.disease
Tropical Diseases
030112 virology
Sciences biomédicales
Co-Infections
biology.protein
Preventive Medicine
Clinical Medicine
business
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PloS one, 15 (12 December, PLoS ONE, 15(12 December):e0244800. Public Library of Science, PLoS ONE, PLoS ONE, Vol 15, Iss 12, p e0244800 (2020)
- Accession number :
- edsair.doi.dedup.....875a91a755fb4e01fab077716321d9ab