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Retracted: Inhibition of SNKāSPAR signaling pathway promotes the restoration of motor function in a rat model of ischemic stroke
- Source :
- Journal of Cellular Biochemistry. 119:1093-1110
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- This study aimed to investigate the effects of SPAR signaling pathway on the restoration of motor function in ischemic stroke (IS). Sprague-Dawley male rats were separated into the control and sham groups, as well as the group for middle cerebral artery occlusion (MCAO) model establishment. Successfully established rat ischemic models were randomly divided into model, SNKMCAO-del and pcDNA3.1-SNK groups. The evaluation of motor function among the rats in each group was assessed using a balance beam, a screen test and the Garcia scoring method. CatWalk gait analysis was employed to evaluate the effect of the SNK signaling pathway on rat motor function. Triphenyltetrazolium chloride (TTC) and TUNEL staining were techniques were utilized for cerebral infarction (CI) area as well for hippocampal neuron apoptosis. The quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting methods were performed to detect mRNA and protein expressions of SNK and SPAR. When compared with the model group, the SNKMCAO-del group displayed decreased motor function score and CI area, while contrasting results were observed in the pcDNA3.1-SNK group. According to the results obtained from the CatWalk gait analysis, the SNKMCAO-del group showed a clear improvement compared to the model group whereas the pcDNA3.1-SNK group exhibited poorer results than the model group in the objective parameters of the study, such as movement, speed, running duration, print area, maximal contact area, maximal, mean intensity, and stride length. These findings suggested that SNK gene silencing promotes motor function by inhibiting the SNK-SPAR signaling pathway in rats with ischemic stroke.
- Subjects :
- Male
0301 basic medicine
medicine.medical_specialty
Genetic Vectors
Protein Serine-Threonine Kinases
Screen test
Biochemistry
Brain Ischemia
Rats, Sprague-Dawley
Random Allocation
03 medical and health sciences
0302 clinical medicine
Internal medicine
medicine
Animals
Humans
Gene silencing
Gene Silencing
Gait
Molecular Biology
Balance (ability)
TUNEL assay
business.industry
Cerebral infarction
GTPase-Activating Proteins
Cell Biology
medicine.disease
Rats
Stroke
Disease Models, Animal
030104 developmental biology
Endocrinology
Apoptosis
Gait analysis
Signal transduction
business
030217 neurology & neurosurgery
Signal Transduction
Subjects
Details
- ISSN :
- 10974644 and 07302312
- Volume :
- 119
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....8744e007e130e722e90768faf3455f4f
- Full Text :
- https://doi.org/10.1002/jcb.26278