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Methods for quantifying lysophosphatidic acid in body fluids: A review
- Source :
- Analytical Biochemistry. 453:38-43
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Lysophosphatidic acid (LPA) is a bioactive lipid involved in cellular signal transduction. LPA plays a role in both physiological and pathological processes. Elevated levels of LPA are observed in the plasma of patients with epithelial ovarian cancer, indicating its potential as a diagnostic marker. Quantification of total LPA can be performed by radioenzymatic, fluorometric, colorimetric, or immunoezymatic assay. Determination of individual LPA molecular species requires the use of capillary electrophoresis, gas chromatography, thin layer chromatography, liquid chromatography, or a matrix-assisted laser desorption/ionization time-of-flight method connected to an appropriate detection system.
- Subjects :
- Biophysics
Enzyme-Linked Immunosorbent Assay
Carcinoma, Ovarian Epithelial
Biochemistry
chemistry.chemical_compound
Capillary electrophoresis
Lysophosphatidic acid
Biomarkers, Tumor
Humans
Fluorometry
Epithelial ovarian cancer
Neoplasms, Glandular and Epithelial
Molecular Biology
Enzyme Assays
Ovarian Neoplasms
Chromatography
Chemistry
Diagnostic marker
Cell Biology
Cellular signal transduction
Thin-layer chromatography
Bioactive lipid
Body Fluids
Colorimetry
lipids (amino acids, peptides, and proteins)
Gas chromatography
Lysophospholipids
biological phenomena, cell phenomena, and immunity
Blood Chemical Analysis
Subjects
Details
- ISSN :
- 00032697
- Volume :
- 453
- Database :
- OpenAIRE
- Journal :
- Analytical Biochemistry
- Accession number :
- edsair.doi.dedup.....874359ce5b18d24bfa69b27d45b1c0be