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Crystal Structures of the TRAF2: cIAP2 and the TRAF1: TRAF2: cIAP2 Complexes: Affinity, Specificity, and Regulation
- Source :
- Molecular Cell. 38(1):101-113
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- TRAF1/2 and cIAP1/2 are members of the TNF receptor associated factor (TRAF) and the inhibitor of apoptosis (IAP) families, respectively. They are critical for both the canonical and the noncanonical NF-κB signaling pathways. Here we report the crystal structures of the TRAF2: cIAP2 and the TRAF1: TRAF2: cIAP2 complexes. A TRAF2 trimer interacts with one cIAP2 both in the crystal and in solution. Two chains of the TRAF2 trimer directly contact cIAP2 and key residues at the interface are confirmed by mutagenesis both in vitro and in cells. TRAF1 and TRAF2 preferentially form the TRAF1: (TRAF2)2 heterotrimer, which interacts with cIAP2 more strongly than TRAF2 alone. In contrast, TRAF1 alone interacts very weakly with cIAP2. Surprisingly, TRAF1 and one chain of TRAF2 in the TRAF1: (TRAF2)2: cIAP2 ternary complex mediate interaction with cIAP2. Because TRAF1 is dramatically upregulated by many stimuli, it may modulate the interaction of TRAF2 with cIAP1/2, which explains previously noted regulatory roles of TRAF1 in TNF signaling.
- Subjects :
- Models, Molecular
TRAF2
Protein Conformation
Ubiquitin-Protein Ligases
Molecular Sequence Data
TRAF1
Trimer
Plasma protein binding
Biology
Crystallography, X-Ray
Inhibitor of apoptosis
Article
Cell Line
Inhibitor of Apoptosis Proteins
Mice
03 medical and health sciences
0302 clinical medicine
Animals
Humans
Amino Acid Sequence
Ternary complex
Molecular Biology
030304 developmental biology
Mice, Knockout
0303 health sciences
Mutagenesis
NF-kappa B
Cell Biology
TNF Receptor-Associated Factor 2
TNF Receptor-Associated Factor 1
Molecular biology
Baculoviral IAP Repeat-Containing 3 Protein
Multiprotein Complexes
030220 oncology & carcinogenesis
Biophysics
Signal transduction
Sequence Alignment
Signal Transduction
Subjects
Details
- ISSN :
- 10972765
- Volume :
- 38
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Molecular Cell
- Accession number :
- edsair.doi.dedup.....87412661bcfb80228825c661944ecb49
- Full Text :
- https://doi.org/10.1016/j.molcel.2010.03.009