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Human haematological and epithelial tumor-derived cell lines express distinct patterns of onco-microRNAs
- Source :
- Cellular and molecular biology (Noisy-le-Grand, France). 63(11)
- Publication Year :
- 2017
-
Abstract
- MicroRNAs post-transcriptionally regulate gene expression thus playing a critical role in a wide range of physiological and pathological processes, including cancer initiation and progression. Moreover, a growing number of evidences highlights that miRNAs themselves are differentially expressed between normal and malignant tissues. In this study, we analysed differences in miRNA expression profile between haematological and epithelial tumor-derived cell lines and explored their role in definying different cancer cells phenotypes. Cancer Focus microRNA PCR Panel was used to analyze eighty-four oncomiRNAs in two human haematological (K562 and HL-60) and in two epithelial (H460 and MCF-7) cancer cell lines. Bioinformatic tools were used to identify miRNA-specific signatures and to discover potentially deregulated pathways. Our analysis led to the identification of i) a large repertoire of miRNAs commonly expressed in the four cell lines, including two equally highly expressed (UPmiRs) and four equally low expressed (DNmiRs); ii) two miRNAs signatures, one associated with the haematological and one with the epithelial cell lines; iii) miRNA signatures specific for the acute or for the chronic myeloid leukemic cells; iv) miRNA signatures specific for the lung or for the breast carcinoma cells. As a whole, these results strengthen the significance of miRNAs profiling in human cancer subtyping, providing the ground for the identification of novel potential biomarkers for specific cancer cell phenotypes.
- Subjects :
- 0301 basic medicine
Myeloid
Breast Neoplasms
HL-60 Cells
Biology
Real-Time Polymerase Chain Reaction
03 medical and health sciences
Cell Line, Tumor
Gene expression
microRNA
medicine
Humans
Gene Expression Profiling
Epithelial Cells
General Medicine
Phenotype
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
MCF-7
Cell culture
Cancer cell
Cancer research
MCF-7 Cells
K562 cells
Subjects
Details
- ISSN :
- 1165158X
- Volume :
- 63
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Cellular and molecular biology (Noisy-le-Grand, France)
- Accession number :
- edsair.doi.dedup.....871c655c26ccfe7852df30842d1dcbc9