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Ultrasmall compact CMOS imaging system for bioluminescence reporter-based live gene expression analysis
- Source :
- Journal of Biomedical Optics
- Publication Year :
- 2021
-
Abstract
- Significance: Gene expression analysis is an important fundamental area of biomedical research. However, live gene expression imaging has proven challenging due to constraints in conventional optical devices and fluorescent reporters. Aim: Our aim is to develop smaller, more cost-effective, and versatile imaging capabilities compared with conventional devices. Bioluminescence reporter-based gene expression analysis was targeted due to its advantages over fluorescence-based imaging. Approach: We created a small compact imaging system using micro-CMOS image sensors (μCIS). The μCIS model had an improved pixel design and a patterned absorption filter array to detect the low light intensity of bioluminescence. Results: The device demonstrated lower dark current, lower temporal noise, and higher sensitivity compared with previous designs. The filter array enabled us to subtract dark current drift and attain a clearer light signal. These improvements allowed us to measure bioluminescence reporter-based gene expression in living mammalian cells. Conclusion: Using our μCIS system for bioluminescence imaging in the future, the device can be implanted in vivo for simultaneous gene expression imaging, behavioral analysis, and optogenetic modulation.
- Subjects :
- Physics
Paper
Pixel
Biomedical Engineering
biophotonics
image sensors
luciferase
bioluminescence
Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Imaging
Biomaterials
Biophotonics
micro-CMOS
Light intensity
Genes, Reporter
Luminescent Measurements
gene expression
Bioluminescence imaging
Bioluminescence
Animals
Image sensor
Preclinical imaging
Dark current
Biomedical engineering
Subjects
Details
- ISSN :
- 15602281
- Volume :
- 26
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of biomedical optics
- Accession number :
- edsair.doi.dedup.....871a122e140d330acd6a74c9f39bbec8