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IDENTIFICATION OF GROUP A COXSACKIEVIRUSES BY IMMUNE ADHERENCE HEMAGGLUTINATION
- Source :
- Japanese Journal of Medical Science and Biology. 36:199-207
- Publication Year :
- 1983
- Publisher :
- Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis, 1983.
-
Abstract
- We investigated to find whether the immune adherence hemagglutination (IAHA) test could be used for identification of group A coxsackieviruses (Cox. A). By using homogenate of suckling mouse torsos infected with each of nine prototype viruses (Cox. A 2, 3, 4, 5, 6, 8, 9, 10 and 16) and 46 isolates as antigens and hyperimmune mouse ascitic fluids to the prototype viruses, we compared IAHA with complement fixation (CF) for serotyping of these viruses. The results of identification tests by IAHA were the same as those by combined use of CF and neutralization tests on all the 46 strains. By CF alone, however, six of 46 strains were not identified because of lower antigen titers; IAHA antigen titers were generally higher by 16-fold or more than CF tests. Furthermore, IAHA had a higher type-specificity than CF; a weak cross-reaction was found by IAHA only between Cox. A 3 and Cox. A 8. Nonspecific reactions encountered in IAHA were reduced more readily by kaolin than fluorocarbon treatment of the torso homogenates. From these results, we conclude that IAHA is an alternative method to CF and neutralization for serotyping of Cox. A viruses.
- Subjects :
- Serotype
biology
Hemagglutination
business.industry
Complement Fixation Tests
Immune adherence
Hemagglutination Tests
Coxsackievirus
biology.organism_classification
Complement fixation test
Virology
General Biochemistry, Genetics and Molecular Biology
Neutralization
Mice
Titer
Antigen
Animals
Medicine
Serotyping
Kaolin
business
Chlorofluorocarbons, Methane
Enterovirus
Subjects
Details
- ISSN :
- 18842828 and 00215112
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Japanese Journal of Medical Science and Biology
- Accession number :
- edsair.doi.dedup.....86f20152357f22c187585ee8371a388f