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'Global' cell replacement is feasible via neural stem cell transplantation: Evidence from the dysmyelinated shiverer mouse brain
- Publication Year :
- 1999
- Publisher :
- The National Academy of Sciences, 1999.
-
Abstract
- Many diseases of the central nervous system (CNS), particularly those of genetic, metabolic, or infectious/inflammatory etiology, are characterized by “global” neural degeneration or dysfunction. Therapy might require widespread neural cell replacement, a challenge not regarded conventionally as amenable to neural transplantation. Mouse mutants characterized by CNS-wide white matter disease provide ideal models for testing the hypothesis that neural stem cell transplantation might compensate for defective neural cell types in neuropathologies requiring cell replacement throughout the brain. The oligodendrocytes of the dysmyelinated shiverer ( shi ) mouse are “globally” dysfunctional because they lack myelin basic protein (MBP) essential for effective myelination. Therapy, therefore, requires widespread replacement with MBP-expressing oligodendrocytes. Clonal neural stem cells transplanted at birth—using a simple intracerebroventricular implantation technique—resulted in widespread engraftment throughout the shi brain with repletion of MBP. Accordingly, of the many donor cells that differentiated into oligodendroglia—there appeared to be a shift in the fate of these multipotent cells toward an oligodendroglial fate—a subgroup myelinated up to 52% (mean = ≈40%) of host neuronal processes with better compacted myelin of a thickness and periodicity more closely approximating normal. A number of recipient animals evinced decrement in their symptomatic tremor. Therefore, “global” neural cell replacement seems feasible for some CNS pathologies if cells with stem-like features are used.
- Subjects :
- Neurons
Multidisciplinary
biology
Stem Cells
Neural degeneration
Cell Differentiation
Biological Sciences
Neural stem cell
Myelin basic protein
Transplantation
Neuroepithelial cell
Mice
Mice, Neurologic Mutants
Oligodendroglia
Immunology
biology.protein
Animals
Stem cell
Neuroscience
Neural cell
Adult stem cell
Demyelinating Diseases
Stem Cell Transplantation
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....86ea08f253c75daebfba2029c111d324