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Enrichment of interleukin-2-responsive natural killer progenitors in human bone marrow
- Source :
- Blood. 81(7)
- Publication Year :
- 1993
-
Abstract
- Natural killer (NK) cells can be cultured in interleukin-2 (IL-2)- containing medium from selected human bone marrow (BM) cells obtained after the elimination of mature T and NK cells. To isolate and characterize IL-2-responsive NK progenitors in the selected BM cells, we investigated the expression of IL-2 receptors (IL-2R) on these cells. Neither CD25 (IL-2R alpha) nor IL-2R beta antigen was observed on the selected BM cells before culture. However, CD25+ cells without detectable levels of IL-2R beta antigen appeared 24 hours after culture in IL-2-containing medium. Cells were sorted from each fraction of the selected BM cells 24 hours after culture after staining with anti-CD33, anti-CD34, and anti-CD25 monoclonal antibodies. The generation of NK cells (CD3- CD56+ cells) and NK activity were observed only from the CD33-/CD34-/CD25+ cell fraction after culture in IL-2-containing medium. The frequency of IL-2-responsive NK progenitors relative to the fraction was 1/231 (95% confidence range, 1/156 to 1/289), which corresponded to the frequency relative to the total number of selected BM cells when the frequency relative to the CD33-/CD34-/CD25+ cell- fraction was converted according to the percentage of these cells in the total number of selected BM cells. These results indicated that IL- 2-responsive NK progenitors were enriched in the CD33-/CD34-/CD25+ cell fraction.
- Subjects :
- Interleukin 2
Cellular differentiation
Immunology
Fluorescent Antibody Technique
Bone Marrow Cells
Biology
Lymphocyte Activation
Biochemistry
Natural killer cell
Interleukin 21
Leukocyte Count
Antigen
Bone Marrow
medicine
Humans
IL-2 receptor
Cells, Cultured
Cell Differentiation
Cell Biology
Hematology
Hematopoietic Stem Cells
Molecular biology
Killer Cells, Natural
medicine.anatomical_structure
Cell culture
Interleukin-2
Bone marrow
medicine.drug
Subjects
Details
- ISSN :
- 00064971
- Volume :
- 81
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....86e4d836ffe94edd1a8e394cffc9f430