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Use of the Chinchilla model to evaluate the vaccinogenic potential of the Moraxella catarrhalis filamentous hemagglutinin-like proteins MhaB1 and MhaB2
- Source :
- PLoS ONE, Vol 8, Iss 7, p e67881 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Moraxella catarrhalis causes significant health problems, including 15–20% of otitis media cases in children and ∼10% of respiratory infections in adults with chronic obstructive pulmonary disease. The lack of an efficacious vaccine, the rapid emergence of antibiotic resistance in clinical isolates, and high carriage rates reported in children are cause for concern. In addition, the effectiveness of conjugate vaccines at reducing the incidence of otitis media caused by Streptococcus pneumoniae and nontypeable Haemophilus influenzae suggest that M. catarrhalis infections may become even more prevalent. Hence, M. catarrhalis is an important and emerging cause of infectious disease for which the development of a vaccine is highly desirable. Studying the pathogenesis of M. catarrhalis and the testing of vaccine candidates have both been hindered by the lack of an animal model that mimics human colonization and infection. To address this, we intranasally infected chinchilla with M. catarrhalis to investigate colonization and examine the efficacy of a protein-based vaccine. The data reveal that infected chinchillas produce antibodies against antigens known to be major targets of the immune response in humans, thus establishing immune parallels between chinchillas and humans during M. catarrhalis infection. Our data also demonstrate that a mutant lacking expression of the adherence proteins MhaB1 and MhaB2 is impaired in its ability to colonize the chinchilla nasopharynx, and that immunization with a polypeptide shared by MhaB1 and MhaB2 elicits antibodies interfering with colonization. These findings underscore the importance of adherence proteins in colonization and emphasize the relevance of the chinchilla model to study M. catarrhalis–host interactions.
- Subjects :
- Filamentous haemagglutinin adhesin
lcsh:Medicine
Pathogenesis
medicine.disease_cause
Bacterial Adhesion
Haemophilus influenzae
Moraxella catarrhalis
Chinchilla
Nasopharynx
Gram Negative
lcsh:Science
Immune Response
Vaccines
0303 health sciences
Multidisciplinary
biology
Vaccination
Animal Models
Antibodies, Bacterial
Bacterial Pathogens
3. Good health
Host-Pathogen Interaction
Hemagglutinins
Streptococcus pneumoniae
Medical Microbiology
Bacterial Vaccines
Host-Pathogen Interactions
Medicine
medicine.symptom
Research Article
Moraxellaceae Infections
Blotting, Western
Immunology
Microbiology
03 medical and health sciences
Model Organisms
Antibiotic resistance
Bacterial Proteins
Cell Line, Tumor
Vaccine Development
medicine
Animals
Humans
Adhesins, Bacterial
Biology
Immunity to Infections
Microbial Pathogens
030304 developmental biology
030306 microbiology
lcsh:R
Immunity
Immune Defense
biology.organism_classification
Bacterial adhesin
Disease Models, Animal
Otitis Media
Otitis
Infectious disease (medical specialty)
Mutation
Humoral Immunity
Clinical Immunology
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....86c556c5c821484f63f96d18634899b5