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Circ_0008532 promotes bladder cancer progression by regulation of the miR-155-5p/miR-330-5p/MTGR1 axis
- Source :
- Journal of Experimental & Clinical Cancer Research : CR, Journal of Experimental & Clinical Cancer Research, Vol 39, Iss 1, Pp 1-12 (2020)
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Background Circular RNAs (circRNAs) have been associated with bladder cancer (BC), but the specific underlying molecular mechanism of their association with BC development has not been fully explored. Methods Levels of Circ_0008532, MTGR1 and miR-155-5p/miR-330-5p in bladder cancer cell lines and tissues were determined with quantitative real-time PCR and western blotting assays. In vitro and in vivo assays were performed to investigate the function of circ_0008532 in tumorigenesis in bladder cancer cells. The relationships of Circ_0008532, MTGR1 and miR-155-5p/miR-330-5p were predicted using bioinformatic tools and verified by RNA-FISH, RIP and luciferase assays. The effects of circ_0008532 on the Notch signaling pathway were determined by GSEA analysis and western blotting assay. Results We found that circ_0008532 is upregulated in BC cell lines and tissues. Moreover, overexpression of circ_0008532 promotes, and silencing of circ_0008532 inhibits the capacity for invasive in BC cells. In addition, circ_0008532 can directly interact with miR-155-5p and miR-330-5p as an miRNA sponge which mediates the expression of the miR-155-5p/miR-330-5p target gene MTGR1 and downstream Notch signaling. Conclusions Circ_0008532 may act as an oncogene in BC through a novel circ_0008532/miR-155-5p, miR-330-5p /MTGR1/Notch pathway axis, which in turn may provide potential biomarkers and a therapeutic target for the management of bladder cancer.
- Subjects :
- 0301 basic medicine
Cancer Research
Notch
Notch signaling pathway
Mice, Nude
Apoptosis
Biology
medicine.disease_cause
lcsh:RC254-282
miR-155
Mice
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
Cell Movement
Biomarkers, Tumor
Tumor Cells, Cultured
medicine
Animals
Humans
Gene silencing
Neoplasm Invasiveness
Cell Proliferation
Mice, Inbred BALB C
Bladder cancer
Oncogene
MTGR1
Research
Circ_0008532
RNA, Circular
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
Prognosis
medicine.disease
Xenograft Model Antitumor Assays
Gene Expression Regulation, Neoplastic
Repressor Proteins
Survival Rate
Blot
MicroRNAs
030104 developmental biology
Urinary Bladder Neoplasms
Oncology
030220 oncology & carcinogenesis
Disease Progression
Cancer research
Carcinogenesis
Subjects
Details
- ISSN :
- 17569966
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental & Clinical Cancer Research
- Accession number :
- edsair.doi.dedup.....86bf910f3a43e66453003d76be2e2f82