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Evaluation of ε-polylysine as antimicrobial alternative for liquid-stored boar semen
- Source :
- Theriogenology. 130:146-156
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- e-polylysine (e-PL) has potent antibacterial effects and is often used in the food industry. However, no studies have clarified the antibacterial effects of e-PL during storage of boar semen. In this study, boar semen samples were diluted with BTS buffer supplemented with different concentrations (0, 0.04, 0.08, 0.16, 0.32, 0.64, and 1.28 g/L) of e-PL and different combinations of e-PL plus gentamicin during liquid storage at 17 °C for 5 days. Bacterial concentrations, bacterial community compositions, sperm quality parameters, and in vitro fertilization (IVF) were evaluated in order to analyze the antibacterial effects of these parameters during boar semen preservation. The results indicated that the optimum concentration of e-PL was 0.16 g/L, which significantly improved sperm quality parameters, including sperm motility, plasma membrane integrity, mitochondrial membrane potential, and acrosome integrity, and changed bacterial proliferation and composition (P
- Subjects :
- Male
endocrine system
Time Factors
Swine
medicine.medical_treatment
Specimen Handling
Andrology
03 medical and health sciences
0302 clinical medicine
Human fertilization
Anti-Infective Agents
Food Animals
Semen
Capacitation
medicine
Animals
Polylysine
Small Animals
Acrosome
Sperm motility
030219 obstetrics & reproductive medicine
In vitro fertilisation
Dose-Response Relationship, Drug
urogenital system
Equine
Chemistry
0402 animal and dairy science
04 agricultural and veterinary sciences
Antimicrobial
040201 dairy & animal science
Sperm
Sperm Motility
Animal Science and Zoology
Gentamicin
Semen Preservation
medicine.drug
Subjects
Details
- ISSN :
- 0093691X
- Volume :
- 130
- Database :
- OpenAIRE
- Journal :
- Theriogenology
- Accession number :
- edsair.doi.dedup.....8698c1107bea1d18a7396791a795908f