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A novel lamin A/C gene missense mutation (445 V > E) in immunoglobulin-like fold associated with left ventricular non-compaction
- Source :
- Europace. 18:617-622
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- Aims Two LMNA mutations (R644C and R190W) have been associated with familial and sporadic left ventricular non-compaction (LVNC). However, the mechanisms underlying these associations have not been elucidated. Methods and results Genomic DNA was isolated from peripheral blood leucocytes and analysed by direct sequencing. Human embryonic kidney 293 cells were transfected with either wild type or mutant LMNA and SCN5A for whole-cell patch-clamp experiment and fluorescence microscopy. Point mutation modeling for mutant LMNA was also performed. One novel LVNC-associated mutation (V445E) in β2 sheet of immunoglobulin (Ig)-like fold was found in the proband and his father. We also found that the peak current of sodium channel was markedly reduced in mutant LMNA compared with WT while the activation, inactivation, and recovery curves were not significantly altered. The mutant lamin A/C were aggregated into multiple highlighted particles. Three β sheets and multiple side chains in Ig-like fold were altered due to the replacement of a valine by glutamic acid. Conclusion Our data associated a novel lamin A/C mutation (V445E) with a sudden death form of familial LVNC. The reduced sodium current in mutant LMNA may account for the advent of malignant ventricular arrhythmias. The altered structures of three β sheets and side chains may partially explain the aggregation of lamin A/C protein subjacent to the nuclear envelope.
- Subjects :
- Male
Models, Molecular
0301 basic medicine
Protein Folding
DNA Mutational Analysis
Mutant
030204 cardiovascular system & hematology
medicine.disease_cause
Membrane Potentials
LMNA
Electrocardiography
0302 clinical medicine
Missense mutation
Genetics
Mutation
Isolated Noncompaction of the Ventricular Myocardium
integumentary system
Valine
Lamin Type A
Phenotype
Echocardiography
Ventricular Fibrillation
embryonic structures
Cardiology and Cardiovascular Medicine
Heterozygote
congenital, hereditary, and neonatal diseases and abnormalities
Adolescent
Mutation, Missense
Glutamic Acid
Transfection
Sudden death
Protein Aggregates
Structure-Activity Relationship
Young Adult
03 medical and health sciences
Physiology (medical)
medicine
Humans
Genetic Predisposition to Disease
business.industry
Point mutation
Wild type
Molecular biology
Death, Sudden, Cardiac
HEK293 Cells
030104 developmental biology
Microscopy, Fluorescence
Mutagenesis, Site-Directed
Tachycardia, Ventricular
Protein Conformation, beta-Strand
business
Lamin
Subjects
Details
- ISSN :
- 15322092 and 10995129
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Europace
- Accession number :
- edsair.doi.dedup.....868321a5dca323aabf76e05aec5c83f1