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Acrolein impairs the cholesterol transport functions of high density lipoproteins
- Source :
- PLoS ONE, Vol 10, Iss 4, p e0123138 (2015), PLoS ONE
- Publication Year :
- 2015
- Publisher :
- Public Library of Science (PLoS), 2015.
-
Abstract
- High density lipoproteins (HDL) are considered athero-protective, primarily due to their role in reverse cholesterol transport, where they transport cholesterol from peripheral tissues to the liver for excretion. The current study was designed to determine the impact of HDL modification by acrolein, a highly reactive aldehyde found in high abundance in cigarette smoke, on the cholesterol transport functions of HDL. HDL was chemically-modified with acrolein and immunoblot and mass spectrometry analyses confirmed apolipoprotein crosslinking, as well as acrolein adducts on apolipoproteins A-I and A-II. The ability of acrolein-modified HDL (acro-HDL) to serve as an acceptor of free cholesterol (FC) from COS-7 cells transiently expressing SR-BI was significantly decreased. Further, in contrast to native HDL, acro-HDL promotes higher neutral lipid accumulation in murine macrophages as judged by Oil Red O staining. The ability of acro-HDL to mediate efficient selective uptake of HDL-cholesteryl esters (CE) into SR-BI-expressing cells was reduced compared to native HDL. Together, the findings from our studies suggest that acrolein modification of HDL produces a dysfunctional particle that may ultimately promote atherogenesis by impairing functions that are critical in the reverse cholesterol transport pathway.
- Subjects :
- Apolipoprotein B
High density
lcsh:Medicine
030204 cardiovascular system & hematology
Excretion
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Chlorocebus aethiops
Oil Red O
Animals
Acrolein
lcsh:Science
030304 developmental biology
0303 health sciences
Multidisciplinary
COS cells
biology
Cholesterol
Macrophages
Reverse cholesterol transport
lcsh:R
nutritional and metabolic diseases
Biological Transport
Mice, Inbred C57BL
chemistry
Biochemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
COS Cells
biology.protein
lipids (amino acids, peptides, and proteins)
lcsh:Q
Chromatography, Thin Layer
Lipoproteins, HDL
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 10
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....8680a27be818239b3ed948822dd10c56