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The 3D OrbiSIMS—label-free metabolic imaging with subcellular lateral resolution and high mass-resolving power
- Source :
- Nature Methods. 14:1175-1183
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- © 2017 Nature America, Inc., part of Springer Nature. All rights reserved. We report the development of a 3D OrbiSIMS instrument for label-free biomedical imaging. It combines the high spatial resolution of secondary ion mass spectrometry (SIMS; under 200 nm for inorganic species and under 2 μm for biomolecules) with the high mass-resolving power of an Orbitrap (>240,000 at m/z 200). This allows exogenous and endogenous metabolites to be visualized in 3D with subcellular resolution. We imaged the distribution of neurotransmitters - gamma-aminobutyric acid, dopamine and serotonin - with high spectroscopic confidence in the mouse hippocampus. We also putatively annotated and mapped the subcellular localization of 29 sulfoglycosphingolipids and 45 glycerophospholipids, and we confirmed lipid identities with tandem mass spectrometry. We demonstrated single-cell metabolomic profiling using rat alveolar macrophage cells incubated with different concentrations of the drug amiodarone, and we observed that the upregulation of phospholipid species and cholesterol is correlated with the accumulation of amiodarone.
- Subjects :
- 0301 basic medicine
Serotonin
Resolution (mass spectrometry)
Dopamine
Phospholipid
Amiodarone
Glycerophospholipids
Tandem mass spectrometry
Mass spectrometry
Orbitrap
Hippocampus
Sensitivity and Specificity
01 natural sciences
Biochemistry
law.invention
Mice
03 medical and health sciences
chemistry.chemical_compound
Imaging, Three-Dimensional
Tandem Mass Spectrometry
law
Macrophages, Alveolar
Animals
Metabolomics
Molecular Biology
Cells, Cultured
gamma-Aminobutyric Acid
Drug discovery, Imaging, Mass spectrometry, Metabolomics
Sulfoglycosphingolipids
010401 analytical chemistry
Equipment Design
Cell Biology
Subcellular localization
Molecular Imaging
0104 chemical sciences
Secondary ion mass spectrometry
Matrix-assisted laser desorption/ionization
030104 developmental biology
chemistry
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Biophysics
Female
Subcellular Fractions
Biotechnology
Subjects
Details
- ISSN :
- 15487105 and 15487091
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Nature Methods
- Accession number :
- edsair.doi.dedup.....8678987b5c985672e48ae22c20695052