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Enhancing Effects ofScutellaria baicalensisand Some of its Constituents on TGF-β1 Gene Expression in RAW 264.7 Murine Macrophage Cell Line
- Source :
- Planta Medica. 71:440-445
- Publication Year :
- 2005
- Publisher :
- Georg Thieme Verlag KG, 2005.
-
Abstract
- Transforming growth factor beta1 (TGF-beta1) has been implicated as an inhibitor of cell proliferation and a potent inducer of apoptosis. Scutellaria baicalensis Georgi (SbG) has been widely used in Asia and recent investigations have shown that SbG has anticancer, antiviral, and anti-inflammatory effects. The aim of this study is to investigate the modulatory effect of SbG on TGF-beta1 gene expression. By using RAW 264.7 cell line as an in vitro model, the effects of SbG on TGF-beta1 gene expression were evaluated by ELISA, reverse-transcription polymerase chain reaction (RT-PCR) and quantitative PCR. Many inhibitors such as mitogen-activated protein kinase (MAPK) inhibitor (PD98059), p38-MAPK inhibitor (SB203580), NF-kappaB inhibitor (aspirin) and protein kinase C inhibitor (H7) were used to determine the possible signal transduction pathways. The results showed that crude extracts of SbG as well as its pure compounds, baicalin, baicalein and chrysin up-regulated TGF-beta1 gene expression on RAW 264.7 cells in a concentration-dependent manner. However, the flavonoid of SbG, wogonin, did not up-regulate TGF-beta1 expression on gene and protein levels on RAW264.7 cells. The facts that aspirin and H7 but not PD98059 and SB203580 blocked the enhancing effect suggested that NF-kappaB and PKC might be involved in baicalin-enhanced TGF-beta1 gene expression. We conclude that SbG up-regulates TGF-beta1 gene expression on RAW264.7 cells through NF-kappaB and PKC pathways and this might provide evidence to explain the therapeutic effect and potential adverse effects on the clinical use of Scutellaria baicalensis Georgi.
- Subjects :
- Pharmaceutical Science
Enzyme-Linked Immunosorbent Assay
Pharmacology
Analytical Chemistry
Mice
chemistry.chemical_compound
Wogonin
Drug Discovery
Gene expression
Animals
Immunologic Factors
Protein kinase A
Lymphotoxin-alpha
Protein kinase C
DNA Primers
Regulation of gene expression
Dose-Response Relationship, Drug
biology
Plant Extracts
Reverse Transcriptase Polymerase Chain Reaction
Macrophages
Organic Chemistry
biology.organism_classification
Baicalein
Gene Expression Regulation, Neoplastic
Complementary and alternative medicine
chemistry
Flavanones
Immunology
Molecular Medicine
Scutellaria baicalensis
Signal transduction
Phytotherapy
Signal Transduction
Subjects
Details
- ISSN :
- 14390221 and 00320943
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Planta Medica
- Accession number :
- edsair.doi.dedup.....867820a2acf6914b245f8f2764338ffa